Multiple In vitro biological effects of phenolic compounds from Morus alba root bark
Jazyk angličtina Země Irsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31610262
DOI
10.1016/j.jep.2019.112296
PII: S0378-8741(19)33729-8
Knihovny.cz E-zdroje
- Klíčová slova
- Anti-inflammatory, Antibacterial, Antiviral, Docking, Mulberry, Phenolic,
- MeSH
- buněčné linie MeSH
- diabetes mellitus farmakoterapie metabolismus MeSH
- glukosa metabolismus MeSH
- glykogen metabolismus MeSH
- hypoglykemika farmakologie terapeutické užití MeSH
- inzulinová rezistence MeSH
- játra účinky léků metabolismus MeSH
- kosterní svaly účinky léků metabolismus MeSH
- krysa rodu Rattus MeSH
- listy rostlin MeSH
- membránový potenciál mitochondrií účinky léků MeSH
- Morus * MeSH
- myši MeSH
- proteinkinasy aktivované AMP metabolismus MeSH
- reaktivní formy kyslíku metabolismus MeSH
- rostlinné extrakty farmakologie terapeutické užití MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- glukosa MeSH
- glykogen MeSH
- hypoglykemika MeSH
- proteinkinasy aktivované AMP MeSH
- reaktivní formy kyslíku MeSH
- rostlinné extrakty MeSH
ETHNOPHARMACOLOGICAL RELEVANCE: Morus alba L. is used in traditional Chinese medicine for the treatment of various diseases, including bacterial infections and inflammation. As a rich source of phenolic compounds, the plant is an object of many phytochemical and pharmacological studies. AIM OF THE STUDY: The aim of the study was to isolate and evaluate possible parallel antiviral, antibacterial, and anti-inflammatory activities of phenolic mulberry compounds. MATERIALS AND METHODS: Extensive chromatographic separation of mulberry root bark extract and in vitro biological screening of 26 constituents identified promising candidates for further pharmacological research. Selected compounds were screened for anti-infective and anti-inflammatory activities. Antiviral activity was determined by the plaque number reduction assay and by the titer reduction assay, antibacterial using broth microdilution method, and anti-inflammatory activity using COX Colorimetric inhibitor screening assay kit. One compound was evaluated in vivo in carrageenan-induced paw-edema in mice. RESULTS: Five prenylated compounds 1, 2, 8, 9, and 11, together with a simple phenolic ester 13, exhibited inhibitory activity against the replication of herpes simplex virus 1 (HSV-1) or herpes simplex virus 2 (HSV-2), with IC50 values ranging from 0.64 to 1.93 μg/mL, and EC50 values 0.93 and 1.61 μg/mL. Molecular docking studies demonstrated the effects of the active compounds by targeting HSV-1 DNA polymerase and HSV-2 protease. In antibacterial assay, compounds 1, 4, 11, and 17 diminished the growth of all of the Gram-positive strains tested, with MIC values of 1-16 μg/mL. The anti-inflammatory ability of several compounds to inhibit cyclooxygenase 2 (COX-2) was tested in vitro, and compound 16 displayed greater activity than the indomethacin, positive control. Mulberrofuran B (11) showed anti-inflammatory activity in vivo against carrageenan-induced paw-edema in mice. CONCLUSIONS: Experimental investigation showed promising antiviral, antibacterial, and/or anti-inflammatory activities of the phenolic mulberry constituents, often with multiple inhibitory effects that might be used as a potential source of new medicine.
Citace poskytuje Crossref.org
Natural Phenolics Disrupt Microbial Communication by Inhibiting Quorum Sensing
Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing
Prenylated Flavonoids in Topical Infections and Wound Healing
Berberine in Human Oncogenic Herpesvirus Infections and Their Linked Cancers
Natural Products-Derived Chemicals: Breaking Barriers to Novel Anti-HSV Drug Development
