The membrane-embedded FtsH proteases found in bacteria, chloroplasts, and mitochondria are involved in diverse cellular processes including protein quality control and regulation. The genome of the model cyanobacterium Synechocystis sp PCC 6803 encodes four FtsH homologs designated FtsH1 to FtsH4. The FtsH3 homolog is present in two hetero-oligomeric complexes: FtsH2/3, which is responsible for photosystem II quality control, and the essential FtsH1/3 complex, which helps maintain Fe homeostasis by regulating the level of the transcription factor Fur. To gain a more comprehensive insight into the physiological roles of FtsH hetero-complexes, we performed genome-wide expression profiling and global proteomic analyses of Synechocystis mutants conditionally depleted of FtsH3 or FtsH1 grown under various nutrient conditions. We show that the lack of FtsH1/3 leads to a drastic reduction in the transcriptional response to nutrient stress of not only Fur but also the Pho, NdhR, and NtcA regulons. In addition, this effect is accompanied by the accumulation of the respective transcription factors. Thus, the FtsH1/3 complex is of critical importance for acclimation to iron, phosphate, carbon, and nitrogen starvation in Synechocystis.plantcell;31/12/2912/FX1F1fx1.

Centre Algatech Institute of Microbiology of the Czech Academy of Sciences Třeboň 379 81 Czech Republic

ChELSI Institute Department of Chemical and Biological Engineering University of Sheffield Sheffield S1 3JD United Kingdom

Department of Molecular Biology and Biotechnology University of Sheffield Sheffield S10 2TN United Kingdom

Faculty of Science University of South Bohemia České Budějovice 370 05 Czech Republic

Freiburg Institute for Advanced Studies University of Freiburg Albertstrße 19 D 79104 Freiburg Germany

Genetics and Experimental Bioinformatics Faculty of Biology University of Freiburg D 79104 Freiburg Germany

School of Biomedical Sciences Institute of Translational and Stratified Medicine Faculty of Medicine and Dentistry University of Plymouth Plymouth PL6 8BU United Kingdom

Systems Biology and Bioinformatics Laboratory University of Algarve Campus de Gambelas 8005 139 Faro Portugal

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The Role of FtsH Complexes in the Response to Abiotic Stress in Cyanobacteria

FtsH4 protease controls biogenesis of the PSII complex by dual regulation of high light-inducible proteins