Chestnut shells (Italian cultivar "Marrone di Roccadaspide" PGI): Antioxidant activity and chemical investigation with in depth LC-HRMS/MSn rationalization of tannins
Language English Country Canada Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
32036927
DOI
10.1016/j.foodres.2019.108787
PII: S0963-9969(19)30673-8
Knihovny.cz E-resources
- Keywords
- Antioxidant activity, LC-HRMS/MS(n), Shells, Tannins, Triterpenes, “Marrone di Roccadaspide” PGI, “green” extracts,
- MeSH
- Antioxidants analysis MeSH
- Fagaceae chemistry MeSH
- Phenols analysis MeSH
- Flavonoids analysis MeSH
- Glucosides analysis MeSH
- Evaluation Studies as Topic MeSH
- Hydrolyzable Tannins analysis MeSH
- Calibration MeSH
- Ellagic Acid analysis MeSH
- Humans MeSH
- NF-kappa B metabolism MeSH
- Nuts chemistry MeSH
- Proanthocyanidins analysis MeSH
- Cell Proliferation MeSH
- Rationalization * MeSH
- Reactive Oxygen Species MeSH
- Plant Extracts analysis MeSH
- Tandem Mass Spectrometry MeSH
- Tannins analysis MeSH
- Triterpenes analysis MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Italy MeSH
- Names of Substances
- Antioxidants MeSH
- bartogenic acid MeSH Browser
- Phenols MeSH
- Flavonoids MeSH
- Glucosides MeSH
- Hydrolyzable Tannins MeSH
- Ellagic Acid MeSH
- NF-kappa B MeSH
- Proanthocyanidins MeSH
- Reactive Oxygen Species MeSH
- Plant Extracts MeSH
- Tannins MeSH
- Triterpenes MeSH
In Italy a particularly valuable chestnut is "Marrone di Roccadaspide", a protected geographical indication (PGI) product, deriving from a Castanea sativa cultivar, typical of Salerno province in Campania region. As chestnut industrial processes yield a large amount of shell by-products, in this study the possibility to retrain this waste food as potential source of bioactives was investigated. The ability of "Marrone di Roccadaspide" shell MeOH extract to modulate the pro-inflammatory transcriptional factor NF-κB after LPS stimulation, along with the antioxidant activity by a cell-based in vitro test, were evaluated. To correlate the NF-κB inhibition (67.67% at 5 μg/mL) and the strong antioxidant activity to the chemical composition, an analytical approach based on LC-ESI/LTQOrbitrap/MS/MSn along with NMR characterization of isolated compounds was developed. The identification of hydrolysable and condensed tannins, along with flavonoids, phenol glucosides, ellagic acid derivatives, and triterpenoids was accomplished. The most representative compounds were quantitatively analyzed by LC-ESI/QTrap/MS/MS, showing bartogenic acid as the compound occurring in the highest amount (103.08 mg/100 g shells). With the aim to explore the possibility to employ chestnut shells as suitable source of bioactives for the preparation of functional ingredients, the chemical composition and the antioxidant activity of "eco-friendly" extracts (EtOH and EtOH:H2O 7:3) was finally evaluated, showing a high superimposability of the EtOH:H2O (7:3) extract to the MeOH extract.
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