A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.

Department of Plant Physiology Faculty of Biology University of Belgrade Studentski trg 16 11000 Belgrade Serbia

Department of Plant Physiology Institute for Biological Research Siniša Stanković National Institute of Republic of Serbia University of Belgrade Bulevar Despota Stefana 142 11060 Belgrade Serbia

Laboratory of Hormonal Regulations in Plants Institute of Experimental Botany of the Czech Academy of Sciences Rozvojová 263 CZ 165 02 Prague 6 Czech Republic

Laboratory of Mass Spectrometry Institute of Experimental Botany of the Czech Academy of Sciences Rozvojová 263 CZ 165 02 Prague 6 Czech Republic

Mining and Metallurgy Institute Zeleni Bulevar 35 19219 Bor Serbia

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