Omega-3 polyunsaturated fatty acids (ω-3PUFAs) are introduced into parenteral nutrition (PN) as hepatoprotective but may be susceptible to the lipid peroxidation while olive oil (OO) is declared more peroxidation resistant. We aimed to estimate how the lipid composition of PN mixture affects plasma and erythrocyte lipidome and the propensity of oxidative stress. A cross-sectional comparative study was performed in a cohort of adult patients who were long-term parenterally administered ω-3 PUFAs without (FO/-, n = 9) or with (FO/OO, n = 13) olive oil and healthy age- and sex-matched controls, (n = 30). Lipoperoxidation assessed as plasma and erythrocyte malondialdehyde content was increased in both FO/- and FO/OO groups but protein oxidative stress (protein carbonyls in plasma) and low redox status (GSH/GSSG in erythrocytes) was detected only in the FO/- subcohort. The lipidome of all subjects receiving ω-3 PUFAs was enriched with lipid species containing ω-3 PUFAs (FO/-˃FO/OO). Common characteristic of all PN-dependent patients was high content of fatty acyl-esters of hydroxy-fatty acids (FAHFAs) in plasma while acylcarnitines and ceramides were enriched in erythrocytes. Plasma and erythrocyte concentrations of plasmanyls and plasmalogens (endogenous antioxidants) were decreased in both patient groups with a significantly more pronounced effect in FO/-. We confirmed the protective effect of OO in PN mixtures containing ω-3 PUFAs.

4th Department of Internal Medicine 1st Faculty of Medicine and General University Hospital Charles University 128 08 Prague Czech Republic

Academy of Sciences of the Czech Republic Institute of Physiology 142 20 Prague Czech Republic

Center of Experimental Medicine Institute for Clinical and Experimental Medicine 140 21 Prague Czech Republic

Department of Physiology Faculty of Science Charles University Prague 128 00 Prague Czech Republic

Diabetology Center Institute for Clinical and Experimental Medicine 140 21 Prague Czech Republic

Institute of Hematology and Blood Transfusion 128 20 Prague Czech Republic

University of Chemistry and Technology 166 28 Prague Czech Republic

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