Acidic pH Is Required for the Multilamellar Assembly of Skin Barrier Lipids In Vitro
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
33675786
DOI
10.1016/j.jid.2021.02.014
PII: S0022-202X(21)00238-4
Knihovny.cz E-resources
- MeSH
- Dermatitis, Atopic pathology MeSH
- Ceramides chemistry metabolism MeSH
- Cholesterol chemistry metabolism MeSH
- X-Ray Diffraction MeSH
- Epidermis chemistry metabolism pathology MeSH
- Hydrogen-Ion Concentration MeSH
- Fatty Acids, Nonesterified chemistry metabolism MeSH
- Humans MeSH
- Fatty Acids MeSH
- Microscopy, Atomic Force MeSH
- Infant, Newborn MeSH
- Permeability MeSH
- Water Loss, Insensible * MeSH
- Aged MeSH
- Spectroscopy, Fourier Transform Infrared MeSH
- Age Factors MeSH
- Check Tag
- Humans MeSH
- Infant, Newborn MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ceramides MeSH
- Cholesterol MeSH
- Fatty Acids, Nonesterified MeSH
- Fatty Acids MeSH
Lipid membrane remodeling belongs to the most fundamental processes in the body. The skin barrier lipids, which are ceramide dominant and highly rigid, must attain an unusual multilamellar nanostructure with long periodicity to restrict water loss and prevent the entry of potentially harmful environmental factors. Our data suggest that the skin acid mantle, apart from regulating enzyme activities and keeping away pathogens, may also be a prerequisite for the multilamellar assembly of the skin barrier lipids. Atomic force microscopy on monolayers composed of synthetic or human stratum corneum lipids showed multilayer formation (approximately 10-nm step height) in an acidic but not in a neutral environment. X-ray diffraction, Fourier transform infrared spectroscopy, and permeability studies showed markedly altered lipid nanostructure and increased water loss at neutral pH compared with that at acidic pH. These findings are consistent with the data on the altered organization of skin lipids and increased transepidermal water loss under conditions such as inadequate skin acidification, for example, in neonates, the elderly, and patients with atopic dermatitis.
Faculty of Chemical Technology University of Chemistry and Technology Prague Prague Czech Republic
Faculty of Pharmacy in Hradec Králové Charles University Hradec Králové Czech Republic
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