Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.

Department of Biophysical Chemistry J Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences Prague Czech Republic

Department of Experimental Neurobiology National Institute of Mental Health Klecany Czech Republic

Department of Philosophy and History of Science Faculty of Science Charles University Prague Czech Republic

Department of Psychiatry and Medical Psychology 3rd Faculty of Medicine Charles University Prague Czech Republic

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