The aim of the study was to evaluate the differences in meat quality of 420 Hubbard JA757 cockerels in relation to the housing system (litter and mobile box) and level of mixed feed (ad libitum [AL], reducing the level by 20% [R20] and 30% [R30]). Three groups of chickens were housed in litter boxes for the entire fattening period (stocking density: 0.094 m2/bird). The other 3 groups were housed in litter boxes until 28 d of age and then relocated into mobile boxes (stocking density: 0.154 m2/bird) on pasture until the end of the experiment at 57 d of age. Restricted groups received a reduced diet level from 29th to 57th d of age. Feed mixture restriction increased the pasture vegetation intake of chickens from 2.63 to 3.50 (R20) and 3.94 g of dry matter/bird/d (R30). Restriction adversely affected the dressing percentage (P < 0.001) and breast yield (P < 0.001), while the leg yield (P < 0.001) was increased with increasing restriction levels. Meat of chickens housed in mobile boxes on a pasture showed lower cooking loss (P < 0.001) and higher redness and yellowness values in the skin (P = 0.030 and P = 0.026; respectively) and meat (P = 0.008 and P < 0.001; respectively). The fragile meat after cooking was observed in chickens reared on litter (P = 0.001). As the level of restriction increased, the number of muscle fibres (P = 0.001) increased, and their cross-sectional area (P = 0.001) and diameter (P = 0.002) decreased. The highest contents of lutein (P = 0.002) and zeaxanthin (P = 0.006) in breast muscle were found in chickens housed in mobile boxes and fed 80% and 70% AL. However, the concentrations of α- and γ-tocopherol (P = 0.006 and P = 0.003) were negatively affected by feed restriction. A 30% reduction in feed level in outdoor housed chickens led to a decrease in oxidative stability (P = 0.024). Feed restriction (R20) in chickens housed in mobile boxes significantly increased the n3 fatty acids content (P = 0.002) and h/H index (P = 0.005) and reduced the n6/n3 ratio (P < 0.001) and atherogenic (P < 0.001) and thrombogenic index (P = 0.003), which possess a health benefits for human. In addition, restriction of mixed feed decreased cholesterol content in breast meat (P = 0.042). It might be concluded that, in terms of meat quality, cereal diet restriction of 20% in medium-growing cockerels housed in mobile boxes on a pasture is beneficial. The higher level of restriction does not lead to further improvement in meat quality indicators.

Department of Nutrition Physiology and Animal Product Quality Institute of Animal Science Czech Republic

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AOAC . 18th ed. Association of Official Analytical Chemists; Gaithersburg: 2005. Official Methods of Analysis.

Castellini C., Dal Bosco A., Mugnai C., Bernardini M. Performance and behaviour of chickens with different growing rate reared according to organic system. Ital. J. Anim. Sci. 2002;1:291–300.

Castellini C., Dal Bosco A., Mugnai C., Pedrazzoli M. Comparison of two chicken genotypes organically reared: oxidative stability and other qualitative traits of the meat. Ital. J. Anim. Sci. 2006;5:29–42.

Chen X.D., Ma Q.G., Tang M.Y., Ji C. Development of breast muscle and meat quality in Arbor Acres broilers, Jingxing 100 crossbred chickens and Beijing fatty chickens. Meat Sci. 2007;77:220–227. PubMed

Chodová D., Tůmová E. Feed restriction and muscle fibre characteristics of pectoralis major in broiler chickens. Scientia Agriculturae Bohemica. 2017;48:8–12.

Czauderna M., Kowalczyk J., Marounek M. The simple and sensitive measurement of malondialdehyde in selected specimens of biological origin and some feed by reversed phase high performance liquid chromatography. J. Chromatogr. B. 2011;879:2251–2258. PubMed

Dal Bosco A., Mungai C., Rossati A., Paoletti A., Caporali S., Castelini C. Effect of range enrichment on performance, behavior and forage intake of free-range chickens. J. Appl. Poult. Res. 2014;23:137–145.

Dal Bosco A., Mugnai C., Mattioli S., Rosati A., Ruggeri S., Ranucci D., Castellini C. Transfer of bioactive compounds from pasture to meat in organic free-range chickens. Poult. Sci. 2016;95:2464–2471. PubMed

Englmaierová M., Skřivan M., Taubner T., Skřivanová V. Performance and meat quality of dual-purpose cockerels of dominant genotype reared on pasture. Animals. 2020;10:387. PubMed PMC

European Committee for Standardization. EN 12822 . European Committee for Standardization; Brussels, Belgium: 2000. Foodstuffs—Determination of Vitamin E by High Performance Liquid Chromatography—Measurement of α-, β-, γ- and δ-Tocopherols.

European Committee for Standardization. EN 12823-1 . European Committee for Standardization; Brussels, Belgium: 2000. Foodstuffs—Determination of Vitamin A by High Performance Liquid Chromatography—Part 1: Measurement of All-Trans-Retinol and 13-cis-Retinol.

Folch J.M., Lees M., Sloane-Stanley G.H. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 1957;226:497–509. PubMed

Froescheis O., Moalli S., Liechti H., Bausch J. Determination of lycopene in tissues and plasma of rats by normal-phase high-performance liquid chromatography with photometric detection. J. Chromatogr. B. 2000;739:291–299. PubMed

Glatz P.C., Ru Y.J., Miao Z.H., Wyatt S.K., Rodda B.J. Integrating poultry into a crop and pasture farming system. Int. J. Poult. Sci. 2005;4:187–191.

Imathiu S. Benefits and food safety concerns associated with consumption of edible insects. NFS Journal. 2020;18:1–11.

Kim Y.B., Kim D.H., Jeong S.B., Lee J.W., Kim T.H., Lee H.G., Lee K.W. Black soldier fly larvae oil as an alternative fat source in broiler nutrition. Poult. Sci. 2020;99:3133–3143. PubMed PMC

Koomkrong N., Theerawatanasirikul S., Boonkaewwan Ch., Jaturasitha S., Kayan A. Breed-related number and size of muscle fibres and their response to carcass quality in chickens. Ital. J. Anim. Sci. 2016;14:4145.

Lei S., Van B. Influence of activity and dietary energy on broiler performance, carcass yield and sensory quality. Br. Poult. Sci. 1997;38:183–189. PubMed

Li Y., Yuan L., Yang X., Ni Y., Xia D., Barth S., Grossmann R., Zhao R.Q. Effect of early feed restriction on myofibre types and expression of growth-related genes in the gastrocnemius muscle of crossbred broiler chickens. Br. J. Nutr. 2007;98:310–319. PubMed

Lorenz C., Grashorn M.A. Comparison of crop and gizzard content of conventional and organic broilers. Page 92 in In Proc. of the 24th World's Poultry Congress; Brazil, Salvador de Bahia; 2012.

Michalczuk M., Zdanowska-Sasiadek Z., Damaziak K., Niemiec J. Influence of indoor and outdoor systems on meat quality of slow-growing chickens. CyTA-J. Food. 2017;15:15–20.

Michalczuk M., Łukasiewicz M., Zdanowska-Sąsiadek Ż., Niemiec J. Comparison of selected quality attributes of chicken meat as affected by rearing systems. Pol. J. Food Nutr. Sci. 2014;64:121–126. 2014.

Michiels J., Tagliabue M.M., Akbarian A., Ovyn A., De Smet S. Oxidative status, meat quality and fatty acid profile of broiler chickens reared under free-range and severely feed-restricted conditions compared with conventional indoor rearing. Avian Biol. Res. 2014;7:74–82.

Mugnai C., Dal Bosco A., Castellini C. Effect of rearing system and season on the performance and egg characteristics of Ancona laying hens. Ital. J. Anim. Sci. 2009;8:175–188.

Omosebi D.J., Adeyemi O.A., Sogunle M.O., Idowu O.M.O., Njoku C.P. Effects of duration and level of feed restriction on performance and meat quality of broiler chickens. Arch. Zootec. 2014;63:611–621.

Ponte P.I.P., Alves S.P., Bessa R.J.B., Ferreira L.M.A., Gama L.T., Bras J.L.A., Fontes C., Prates J.A.M. Influence of pasture intake on the fatty acid composition, and cholesterol, tocopherols, and tocotrienols content in meat from free-range broilers. Poult. Sci. 2008;87:80–88. PubMed

Ponte P.I.P., Prates J.A.M., Crespo J.P., Crespo D.G., Mourão J.L., Alves S.P., Bessa R.J.B., Chaveiro-Soares M.A., Gama L.T., Ferreira L.M.A., Fontes C.M.G.A. Restricting the intake of a cereal-based feed in free-range-pastured poultry: effects on performance and meat quality. Poult. Sci. 2008;87:2032–2042. PubMed

Ponte P.I.P., Rosado C.M.C., Crespo J.P., Crespo D.G., Mourão J.L., Chaveiro-Soares M.A., Mendes I., Gama L.T., Prates J.A.M., Ferreira L.M.A., Fontes C.M.G.A. Pasture intake improves the performance and meat sensory attributes of free-range broilers. Poult. Sci. 2008;87:71–79. PubMed

Raes K., De Smet S., Balcaen A., Claeys E., Demeyer D. Effects of diets rich in N-3 polyunsatured fatty acids on muscle lipids and fatty acids in Belgian Blue double-muscled young bulls. Reprod. Nutr. Dev. 2003;43:331–345. PubMed

Rehfeldt C., Fiedler I., Stickland N.C. Number and size of muscle fibers in relation to meat production. In: Te Pas M.F.W., Haagsman, M.E., Everts, H.P., editors. Pages 1–30 in Muscle Development of Livestock Animals: Physiology, Genetics, and Meat Quality. CAB International; Wallingford, UK: 2004.

Santos-Silva J., Bessa R.J.B., Santos-Silva F. Effects of genotype, feeding system and slaughter weight on the quality of light lambs. Fatty acid composition of meat. Livest. Prod. Sci. 2002;77:187–194.

SAS . SAS Institute; Cary, NC: 2003. SAS/STAT User's Guide. Release 9.3.

Schiavone A., Dabbou S., De Marco M., Cullere M., Biasato I., Biasibetti E., Capucchio M.T., Bergagna S., Dezzutto D., Meneguz M., Gai F., Dalle Zotte A., Gasco L. Black soldier fly larva fat inclusion in finisher broiler chicken diet as an alternative fat source. Animal. 2018;12:2032–2039. PubMed

Skřivan M., Englmaierová M. The deposition of carotenoids and α-tocopherol in hen eggs produced under a combination of sequential feeding and grazing. Anim. Feed Sci. Technol. 2014;190:79–86.

Sossidou E.N., Dal Bosco A., Castelini C., Grashorn M.A. Effects of pasture management on poultry welfare and meat quality in organic poultry production systems. Worlds Poult. Sci. J. 2015;71:375–384.

Spranghers T., Michiels J., Vrancx J., Ovyn A., Eeckhout M., De Clercq P., De Smet S. Gut antimicrobial effects and nutritional value of black soldier fly (Hermetia illucens L.) prepupae for weaned piglets. Anim. Feed Sci. Technol. 2018;235:33–42.

Sun T., Long R.J., Liu Z.Y., Ding W.R., Zhang Y. Aspects of lipid oxidation of meat from free-range broilers consuming a diet containing grasshoppers on alpine steppe of the Tibetan Plateau. Poult. Sci. 2012;91:224–231. PubMed

Sun T., Long R.J., Liu Z.Y. The effect of a diet containing grasshoppers and access to free-range on carcase and meat physicochemical and sensory characteristics in broilers. Br. Poult. Sci. 2013;54:130–137. PubMed

Świątkiewicz S., Świątkiewicz M., Arczewska – Włosek A., Józefiak D. Chitosan and its oligosaccharide derivatives (chito-oligosaccharides) as feed supplements in poultry and swine nutrition. J. Anim. Physiol. Anim. Nutr. 2015;99:1–12. PubMed

Ulbricht T.L.V., Southgate D.A.T. Coronary heart disease: seven dietary factors. Lancet. 1991;338:985–992. PubMed

Yang X., Zhuang J., Rao K., Li X., Zhao R. Effect of early feed restriction on hepatic lipid metabolism and expression of lipogenic genes in broiler chickens. Res. Vet. Sci. 2010;89:438–444. PubMed

Zhang X.Q., Jin Y.M., Badgery W.B., Tana Diet selection and n-3 polyunsaturated fatty acid deposition in lambs as affected by restricted time at pasture. Sci. Rep. 2017;7:15641. PubMed PMC

Zhong C., Nakaue H.S., Hu C.Y., Mirosh L.W. Effect of full food and early food restriction on broiler performance, abdominal fat level, cellularity and fat metabolism in broiler chickens. Poult. Sci. 1995;74:1636–1643. PubMed

Utilization of Wheat with Enhanced Carotenoid Levels and Various Fat Sources in Hen Diets