Metabolomics atlas of oral 13C-glucose tolerance test in mice
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
34644567
DOI
10.1016/j.celrep.2021.109833
PII: S2211-1247(21)01297-3
Knihovny.cz E-resources
- Keywords
- 13C, brown adipose tissue, de novo lipogenesis, glucose tolerance, lipidomics, metabolite cycling, metabolomics, metabolomics atlas, pathway analysis, tracer analysis,
- MeSH
- Biomarkers blood MeSH
- Time Factors MeSH
- Chromatography, Liquid MeSH
- Energy Metabolism * MeSH
- Glucose Tolerance Test * MeSH
- Spectrometry, Mass, Electrospray Ionization MeSH
- Blood Glucose metabolism MeSH
- Lipidomics MeSH
- Lipids blood MeSH
- Metabolome * MeSH
- Metabolomics * MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Tandem Mass Spectrometry MeSH
- Tissue Distribution MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- Blood Glucose MeSH
- Lipids MeSH
Glucose tolerance represents a complex phenotype in which many tissues play important roles and interact to regulate metabolic homeostasis. Here, we perform an analysis of 13C6-glucose tissue distribution, which maps the metabolome and lipidome across 12 metabolically relevant mouse organs and plasma, with integrated 13C6-glucose-derived carbon tracing during oral glucose tolerance test (OGTT). We measure time profiles of water-soluble metabolites and lipids and integrate the global metabolite response into metabolic pathways. During the OGTT, glucose use is turned on with specific kinetics at the organ level, but fasting substrates like β-hydroxybutyrate are switched off in all organs simultaneously. Timeline profiling of 13C-labeled fatty acids and triacylglycerols across tissues suggests that brown adipose tissue may contribute to the circulating fatty acid pool at maximal plasma glucose levels. The GTTAtlas interactive web application serves as a unique resource for the exploration of whole-body glucose metabolism and time profiles of tissue and plasma metabolites during the OGTT.
References provided by Crossref.org
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