The biodeterioration of audio-visual materials is a huge problem, as it can cause incalculable losses. To preserve these cultural heritage objects for future generations, it is necessary to determine the main agents of biodeterioration. This study focuses on identifying fungi, both from the air and smears from photographs and cinematographic films that differ in the type of carrier and binder, using high-throughput sequencing approaches. The alpha diversity measures of communities present on all types of carriers were compared, and a significant difference between cellulose acetate and baryta paper was observed. Next, the locality, type of carrier, and audio-visual material seem to affect the structure of fungal communities. Additionally, a link between the occurrence of the most abundant classes and species on audio-visual materials and air contamination in the archives was proven. In both cases, the most abundant classes were Agariomycetes, Dothideomycetes, and Eurotiomycetes, and approximately half of the 50 most abundant species detected on the audio-visual materials and in the air were identical.

Department of Biochemistry and Microbiology Faculty of Food and Biochemical Technology University of Chemistry and Technology Prague Technická 3 16628 Prague 6 Czech Republic

Department of Chemical Technology of Monument Conservation Faculty of Chemistry and Technology University of Chemistry and Technology Prague Technická 5 16628 Prague 6 Czech Republic

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Bingley G., Verran J. Counts of fungal spores released during inspection of mouldy cinematographic film and determination of the gelatinolytic activity of predominant isolates. Int. Biodeterior. Biodegrad. 2013;84:381–387. doi: 10.1016/j.ibiod.2012.04.006. DOI

Liu Z.J., Zhang Y.H., Zhang F.Y., Hu C.T., Liu G.L., Pan J. Microbial Community Analyses of the Deteriorated Storeroom Objects in the Tianjin Museum Using Culture-Independent and Culture-Dependent Approaches. Front. Microbiol. 2018;9:802. doi: 10.3389/fmicb.2018.00802. PubMed DOI PMC

Cappitelli F., Sorlini C. From papyrus to compact disc: The microbial deterioration of documentary heritage. Crit. Rev. Microbiol. 2005;31:1–10. doi: 10.1080/10408410490884766. PubMed DOI

Sclocchi M.C., Krakova L., Pinzari F., Colaizzi P., Bicchieri M., Sakova N., Pangallo D. Microbial Life and Death in a Foxing Stain: A Suggested Mechanism of Photographic Prints Defacement. Microb. Ecol. 2017;73:815–826. doi: 10.1007/s00248-016-0913-7. PubMed DOI

Borrego S., Guiamet P., de Saravia S.G., Batistini P., Garcia M., Lavin P., Perdomo I. The quality of air at archives and the biodeterioration of photographs. Int. Biodeterior. Biodegrad. 2010;64:139–145. doi: 10.1016/j.ibiod.2009.12.005. DOI

Borrego S., Lavin P., Perdomo I., de Saravia S.G., Guiamet P. Determination of Indoor Air Quality in Archives and Biodeterioration of the Documentary Heritage. ISRN Microbiol. 2012;2012 doi: 10.5402/2012/680598. PubMed DOI PMC

Ciesielski S., Pokoj T., Klimiuk E. Cultivation-Dependent and -Independent Characterization of Microbial Community Producing Polyhydroxyalkanoates from Raw Glycerol. J. Microbiol. Biotechnol. 2010;20:853–861. doi: 10.4014/jmb.0909.09038. PubMed DOI

Tepla B., Demnerova K., Stiborova H. History and microbial biodeterioration of audiovisual materials. J. Cult. Herit. 2020;44:218–228. doi: 10.1016/j.culher.2019.12.009. DOI

Otlewska A., Adamiak J., Gutarowska B. Application of molecular techniques for the assessment of microorganism diversity on cultural heritage objects. Acta Biochim. Pol. 2014;61:217–225. doi: 10.18388/abp.2014_1889. PubMed DOI

Bodor A., Bounedjoum N., Vincze G.E., Kis A.E., Laczi K., Bende G., Szilagyi A., Kovacs T., Perei K., Rakhely G. Challenges of unculturable bacteria: Environmental perspectives. Rev. Environ. Sci. Biotechnol. 2020;19:1–22. doi: 10.1007/s11157-020-09522-4. DOI

Branysova T., Tepla B., Demnerova K., Stiborova H., Durovic M. Biodeterioration of Audio-Visual Materials. Chem. Listy. 2021;115:260–265.

Antonelli F., Esposito A., Galotta G., Petriaggi B.D., Piazza S., Romagnoli M., Guerrieri F. Microbiota in Waterlogged Archaeological Wood: Use of Next-Generation Sequencing to Evaluate the Risk of Biodegradation. Appl. Sci. 2020;10:4636. doi: 10.3390/app10134636. DOI

Torralba M.G., Kuelbs C., Moncera K.J., Roby R., Nelson K.E. Characterizing Microbial Signatures on Sculptures and Paintings of Similar Provenance. Microb. Ecol. 2020;81:1098–1105. doi: 10.1007/s00248-020-01504-x. PubMed DOI PMC

Bai F.Y., Chen X.P., Huang J.Z., Lu Y.S., Dong H.Y., Wu Y.H., Song S.L., Yu J., Bai S., Chen Z., et al. Microbial biofilms on a giant monolithic statue of Buddha: The symbiosis of microorganisms and mosses and implications for bioweathering. Int. Biodeterior. Biodegrad. 2021;156 doi: 10.1016/j.ibiod.2020.105106. DOI

Migliore L., Perini N., Mercuri F., Orlanducci S., Rubechini A., Thaller M.C. Three ancient documents solve the jigsaw of the parchment purple spot deterioration and validate the microbial succession model. Sci. Rep. 2019;9:9. doi: 10.1038/s41598-018-37651-y. PubMed DOI PMC

Szulc J., Ruman T., Karbowska-Berent J., Kozielec T., Gutarowska B. Analyses of microorganisms and metabolites diversity on historic photographs using innovative methods. J. Cult. Herit. 2020;45:101–113. doi: 10.1016/j.culher.2020.04.017. DOI

Kracmarova M., Karpiskova J., Uhlik O., Strejcek M., Szakova J., Balik J., Demnerova K., Stiborova H. Microbial Communities in Soils and Endosphere of Solanum tuberosum L. and their Response to Long-Term Fertilization. Microorganisms. 2020;8:9. doi: 10.3390/microorganisms8091377. PubMed DOI PMC

R Core Team R. A Language and Environment for Statistical Computing in R Foundation for Statistical Computing. R Core Team R; Vienna, Austria: 2017.

Callahan B.J., McMurdie P.J., Rosen M.J., Han A.W., Johnson A.J.A., Holmes S.P. DADA2: High-resolution sample inference from Illumina amplicon data. Nat. Methods. 2016;13:581–583. doi: 10.1038/nmeth.3869. PubMed DOI PMC

UNITE Community UNITE General FASTA Release for Fungi 2. Version 18.11.2018. UNITE Community. 2019. [(accessed on 4 September 2020)]. Available online: https://unite.ut.ee/repository.php.

Oksanen J., Blanchet F.G., Kindlt R., Legendre P., O’Hara R.B., Simpson G.L., Solymos P., Stevens M.H.H., Wagner H. Vegan: Community Ecology Package. R-Package Version 2.5-7. 2019. [(accessed on 26 October 2021)]. Available online: https://cran.r-project.org/web/packages/vegan/index.html.

McMurdie P.J., Holmes S. phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data. PLoS ONE. 2013;8 doi: 10.1371/journal.pone.0061217. PubMed DOI PMC

Vivar I., Borrego S., Ellis G., Moreno D.A., Garcia A.M. Fungal biodeterioration of color cinematographic films of the cultural heritage of Cuba. Int. Biodeterior. Biodegrad. 2013;84:372–380. doi: 10.1016/j.ibiod.2012.05.021. DOI

Buckova M., Puskarova A., Sclocchi M.C., Bicchieri M., Colaizzi P., Pinzari F., Pangallo D. Co-occurrence of bacteria and fungi and spatial partitioning during photographic materials biodeterioration. Polym. Degrad. Stab. 2014;108:1–11. doi: 10.1016/j.polymdegradstab.2014.05.025. DOI

Mazzoli R., Giuffrida M.G., Pessione E. Back to the past: “Find the guilty bug-microorganisms involved in the biodeterioration of archeological and historical artifacts”. Appl. Microbiol. Biotechnol. 2018;102:6393–6407. doi: 10.1007/s00253-018-9113-3. PubMed DOI

Lourenco M.J.L., Sampaio J.P. Microbial deterioration of gelatin emulsion photographs: Differences of susceptibility between black and white and colour materials. Int. Biodeterior. Biodegrad. 2009;63:496–502. doi: 10.1016/j.ibiod.2008.10.011. DOI

Kwasna H., Karbowska-Berent J., Behnke-Borowczyk J. Effect of Fungi on the Destruction of Historical Parchment and Paper Documents. Pol. J. Environ. Stud. 2020;29:2679–2695. doi: 10.15244/pjoes/111236. DOI

Cattaneo B., Chelazzi D., Giorgi R., Serena T., Merlo C., Baglioni P. Physico-chemical characterization and conservation issues of photographs dated between 1890 and 1910. J. Cult. Herit. 2008;9:277–284. doi: 10.1016/j.culher.2008.01.004. DOI

Martucci J.F., Ruseckaite R.A. Biodegradation of three-layer laminate films based on gelatin under indoor soil conditions. Polym. Degrad. Stab. 2009;94:1307–1313. doi: 10.1016/j.polymdegradstab.2009.03.018. DOI

Khoramnejadian S. Microbial Degradation of Starch Based Polypropylene. J. Pure Appl. Microbiol. 2013;7:2857–2860.

Savkovic Z., Stupar M., Unkovic N., Ivanovic Z., Blagojevic J., Vukojevic J., Grbic M.L. In vitro biodegradation potential of airborne Aspergilli and Penicillia. Sci. Nat. 2019;106:8. doi: 10.1007/s00114-019-1603-3. PubMed DOI

Okpalanozie O.E., Adebusoye S.A., Troiano F., Catto C., Ilori M.O., Cappitelli F. Assessment of indoor air environment of a Nigerian museum library and its biodeteriorated books using culture-dependent and -independent techniques. Int. Biodeterior. Biodegrad. 2018;132:139–149. doi: 10.1016/j.ibiod.2018.03.003. DOI

Dannemiller K.C., Weschler C.J., Peccia J. Fungal and bacterial growth in floor dust at elevated relative humidity levels. Indoor Air. 2017;27:354–363. doi: 10.1111/ina.12313. PubMed DOI

Puskarova A., Buckova M., Habalova B., Krakova L., Makova A., Pangallo D. Microbial communities affecting albumen photography heritage: A methodological survey. Sci. Rep. 2016;6:20810. doi: 10.1038/srep20810. PubMed DOI PMC

Grbic M.L., Stupar M., Vukojevic J., Maricic I., Bungur N. Molds in Museum Environments: Biodeterioration of Art Photographs and Wooden Sculptures. Arch. Biol. Sci. 2013;65:955–962. doi: 10.2298/ABS1303955G. DOI

Soffritti I., D’Accolti M., Lanzoni L., Volta A., Bisi M., Mazzacane S., Caselli E. The Potential Use of Microorganisms as Restorative Agents: An Update. Sustainability. 2019;11:3853. doi: 10.3390/su11143853. DOI

Ciferri O. Microbial degradation of paintings. Appl. Environ. Microbiol. 1999;65:879–885. doi: 10.1128/AEM.65.3.879-885.1999. PubMed DOI PMC

Pyzik A., Ciuchcinski K., Dziurzynski M., Dziewit L. The Bad and the Good-Microorganisms in Cultural Heritage Environments-An Update on Biodeterioration and Biotreatment Approaches. Materials. 2021;14:177. doi: 10.3390/ma14010177. PubMed DOI PMC

Borrego S., Molina A., Santana A. Fungi in Archive Repositories Environments and the Deterioration of the Graphics Documents. EC Microbiol. 2017;11:205–226.

Pietrzak K., Puchalski M., Otlewska A., Wrzosek H., Guiamet P., Piotrowska M., Gutarowska B. Microbial diversity of pre-Columbian archaeological textiles and the effect of silver nanoparticles misting disinfection. J. Cult. Herit. 2017;23:138–147. doi: 10.1016/j.culher.2016.07.007. DOI

Rick E.M., Woolnough K.F., Seear P.J., Fairs A., Satchwell J., Richardson M., Monteiro W.R., Craner M., Bourne M., Wardlaw A.J., et al. The airway fungal microbiome in asthma. Clin. Exp. Allergy. 2020;50:1325–1341. doi: 10.1111/cea.13722. PubMed DOI

Sugita T., Suto H., Unno T., Tsuboi R., Ogawa H., Shinoda T., Nishikawa A. Molecular Analysis of Malassezia Microflora on the Skin of Atopic Dermatitis Patients and Healthy Subjects. J. Clin. Microbiol. 2001;39:3486–3490. doi: 10.1128/JCM.39.10.3486-3490.2001. PubMed DOI PMC

Chen C., Ji W., Zhao B. Size-dependent efficiencies of ultrafine particle removal of various filter media. Build. Environ. 2019;160 doi: 10.1016/j.buildenv.2019.106171. DOI

Eckmanns T., Rüden H., Gastmeier P. The Influence of High-Efficiency Particulate Air Filtration on Mortality and Fungal Infection among Highly Immunosuppressed Patients: A Systematic Review. J. Infect. Dis. 2006;193:1408–1418. doi: 10.1086/503435. PubMed DOI

Liu G., Xiao M., Zhang X., Gal C., Chen X., Liu L., Pan S., Wu J., Tang L., Clements-Croome D. A review of air filtration technologies for sustainable and healthy building ventilation. Sustain. Cities Soc. 2017;32:375–396. doi: 10.1016/j.scs.2017.04.011. DOI

Sobral M.M.C., Faria M.A., Cunha S.C., Ferreira I.M.P.L.V.O. Toxicological interactions between mycotoxins from ubiquitous fungi: Impact on hepatic and intestinal human epithelial cells. Chemosphere. 2018;202:538–548. doi: 10.1016/j.chemosphere.2018.03.122. PubMed DOI

Rojas T.I., Aira M.J., Batista A., Cruz I.L., Gonzalez S. Fungal biodeterioration in historic buildings of Havana (Cuba) Grana. 2012;51:44–51. doi: 10.1080/00173134.2011.643920. DOI

Uncovering the microbial diversity of Czech Republic archives: A study of metabolically active airborne microbes

Bacterial Diversity on Historical Audio-Visual Materials and in the Atmosphere of Czech Depositories

Microbial Contamination of Photographic and Cinematographic Materials in Archival Funds in the Czech Republic