Chemical genetics in Silene latifolia elucidate regulatory pathways involved in gynoecium development
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
19-02476Y
Czech Science Foundation
RVO:68081731
Czech Academy of Sciences
PubMed
35045170
DOI
10.1093/jxb/erab538
PII: 6511702
Knihovny.cz E-resources
- Keywords
- HANABA TARANU (GATA18), SUPERMAN-like (SlSUP), Silene latifolia, Androhermaphrodite, dioecy, flower development, gynoecium, sex chromosomes,
- MeSH
- Phenotype MeSH
- Flowers genetics MeSH
- Plants MeSH
- Silene * genetics MeSH
- Ovule genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Dioecious plants possess diverse sex determination systems and unique mechanisms of reproductive organ development; however, little is known about how sex-linked genes shape the expression of regulatory cascades that lead to developmental differences between sexes. In Silene latifolia, a dioecious plant with stable dimorphism in floral traits, early experiments suggested that female-regulator genes act on the factors that determine the boundaries of the flower whorls. To identify these regulators, we sequenced the transcriptome of male flowers with fully developed gynoecia, induced by rapid demethylation in the parental generation. Eight candidates were found to have a positive role in gynoecium promotion, floral organ size, and whorl boundary, and affect the expression of class B MADS-box flower genes. To complement our transcriptome analysis, we closely examined the floral organs in their native state using field emission environmental scanning electron microscopy, and examined the differences between females and androhermaphrodites in their placenta and ovule organization. Our results reveal the regulatory pathways potentially involved in sex-specific flower development in the classical model of dioecy, S. latifolia. These pathways include previously hypothesized and unknown female-regulator genes that act on the factors that determine the flower boundaries, and a negative regulator of anther development, SUPERMAN-like (SlSUP).
References provided by Crossref.org
Dosage compensation evolution in plants: theories, controversies and mechanisms
