Additive manufacturing technology, referred as 3D printing technology, is a growing research field with broad applications from nanosensors fabrication to 3D printing of buildings. Nowadays, the world is dealing with a pandemic and requires the use of simple sensing systems. Here, the strengths of fast screening by a lab-on-a-chip device through electrochemical detection using 3D printing technology for SARS-CoV-2 sensing are combined. This system comprises a PDMS microfluidic channel integrated with an electrochemical cell fully 3D-printed by a 3D printing pen (3D-PP). The 3D-PP genosensor is modified with an ssDNA probe that targeted the N gene sequence of SARS-CoV-2. The sensing mechanism relies on the electro-oxidation of adenines present in ssDNA when in contact with SARS-CoV-2 RNA. The hybridization between ssDNA and target RNA takes a place and ssDNA is desorbed from the genosensor surface, causing a decrease of the sensor signal. The developed SARS-CoV-2/3D-PP genosensor shows high sensitivity and fast response.

Center for Advanced Functional Nanorobots Department of Inorganic Chemistry University of Chemistry and Technology Prague Technicka 5 Prague 6 166 28 Czech Republic

Department of Analytical Sciences Faculty of Sciences Universidad Nacional de Educación a Distancia Madrid E 28040 Spain

Department of Medical Research China Medical University Hospital China Medical University No 91 Hsueh Shih Road Taichung Taiwan

Future Energy and Innovation Laboratory Central European Institute of Technology Brno University of Technology Purkynova 656 123 Brno CZ 616 00 Czech Republic

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Spark-Discharge-Activated 3D-Printed Electrochemical Sensors