Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Mass Spectrometry methods MeSH
- Metal Nanoparticles * chemistry MeSH
- Laser Therapy * MeSH
- Lasers MeSH
- Nanoparticles * MeSH
- Gold chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Gold MeSH
We report a new technique for the digital mapping of biomarkers in tissues based on desorption and counting intact gold nanoparticle (Au NP) tags using infrared laser ablation single-particle inductively coupled plasma mass spectrometry (IR LA SP ICP MS). In contrast to conventional UV laser ablation, Au NPs are not disintegrated during the desorption process due to their low absorption at 2940 nm. A mass spectrometer detects up to 83% of Au NPs. The technique is demonstrated on mapping a proliferation marker, nuclear protein Ki-67, in three-dimensional (3D) aggregates of colorectal carcinoma cells, and the results are compared with confocal fluorescence microscopy and UV LA ICP MS. Precise counting of 20 nm Au NPs with a single-particle detection limit in each pixel by the new approach generates sharp distribution maps of a specific biomarker in the tissue. Advantageously, the desorption of Au NPs from regions outside the tissue is strongly suppressed. The developed methodology promises multiplex mapping of low-abundant biomarkers in numerous biological and medical applications using multielemental mass spectrometers.
Department of Chemistry Faculty of Science Masaryk University 625 00 Brno Czech Republic
Department of Experimental Biology Faculty of Science Masaryk University 625 00 Brno Czech Republic
International Clinical Research Center St Anne's University Hospital 602 00 Brno Czech Republic
References provided by Crossref.org
Digital Immunoassay for Biomarker Detection Based on Single-Particle Laser Ablation ICP MS
Detection of Single Ag Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry
