Among amniote vertebrates, geckos represent a clade with exceptional variability in sex determination; however, only a minority of species of this highly diverse group has been studied in this respect. Here, we describe for the first time a female heterogamety in the genus Paroedura, the group radiated in Madagascar and adjacent islands. We identified homomorphic ZZ/ZW sex chromosomes with a highly heterochromatic W chromosome in Paroedura masobe, Paroedura oviceps, Paroedura karstophila, Paroedura stumpffi, and Paroedura lohatsara. Comparative genomic hybridization (CGH) revealed that female-specific sequences are greatly amplified in the W chromosome of P. lohatsara and that P. gracilis seems to possess a derived system of multiple sex chromosomes. Contrastingly, neither CGH nor heterochromatin visualization revealed differentiated sex chromosomes in the members of the Paroedura picta-Paroedura bastardi-Paroedura ibityensis clade, which is phylogenetically nested within lineages with a heterochromatic W chromosome. As a sex ratio consistent with genotypic sex determination has been reported in P. picta, it appears that the members of the P. picta-P. bastardi-P. ibityensis clade possess homomorphic, poorly differentiated sex chromosomes and may represent a rare example of evolutionary loss of highly differentiated sex chromosomes. Fluorescent in situ hybridization (FISH) with a telomeric probe revealed a telomere-typical pattern in all species and an accumulation of telomeric sequences in the centromeric region of autosomes in P. stumpffi and P. bastardi. Our study adds important information for the greater understanding of the variability and evolution of sex determination in geckos and demonstrates how the geckos of the genus Paroedura provide an interesting model for studying the evolution of the sex chromosomes.

Department of Ecology Faculty of Science Charles University Prague Viničná 7 Praha 2 Prague Czech Republic

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J Exp Biol. 2002 Sep;205(Pt 18):2777-84 PubMed

Bioinformatics. 2009 Jun 1;25(11):1451-2 PubMed

Evolution. 2009 Dec;63(12):3043-9 PubMed

Mol Cytogenet. 2013 Dec 18;6(1):60 PubMed

Cytogenet Genome Res. 2014;142(3):190-6 PubMed

Chromosome Res. 2010 Nov;18(7):787-800 PubMed

Proc Biol Sci. 2003 Feb 7;270(1512):313-21 PubMed

Dev Dyn. 2009 Jan;238(1):100-9 PubMed

Nucleic Acids Res. 1991 Sep 11;19(17):4780 PubMed

Evolution. 2014 Jul;68(7):2079-85 PubMed

Chromosoma. 2009 Feb;118(1):43-51 PubMed

Mol Biol Evol. 2013 Dec;30(12):2725-9 PubMed

BMC Genet. 2011 Oct 20;12:90 PubMed

Physiol Biochem Zool. 2012 Sep-Oct;85(5):491-8 PubMed

Mol Phylogenet Evol. 2008 Jan;46(1):74-81 PubMed

BMC Evol Biol. 2013 Apr 29;13:93 PubMed

Evolution. 2014 Apr;68(4):1027-41 PubMed

Chromosome Res. 2010 Nov;18(7):809-20 PubMed

Genetica. 2008 Oct;134(2):243-9 PubMed

J Evol Biol. 2006 Nov;19(6):1775-84 PubMed

Chromosome Res. 2007;15(6):721-34 PubMed

Chromosome Res. 2009;17(1):91-8 PubMed

Naturwissenschaften. 2008 Mar;95(3):209-15 PubMed

Mol Ecol Resour. 2013 Nov;13(6):1019-32 PubMed

Nat Rev Genet. 2007 Dec;8(12):950-62 PubMed

Sex Dev. 2010;4(1-2):88-103 PubMed

Syst Biol. 2005 Oct;54(5):852-9 PubMed

J Evol Biol. 2010 Apr;23(4):670-7 PubMed

Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18190-5 PubMed

Chromosome Res. 2014 Apr;22(1):35-44 PubMed

J Exp Biol. 2013 May 15;216(Pt 10):1872-80 PubMed

Nature. 2013 Jul 18;499(7458):332-5 PubMed

Genetics. 2008 Jul;179(3):1513-25 PubMed

Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9 PubMed

PLoS Biol. 2013;11(8):e1001643 PubMed

Annu Rev Genomics Hum Genet. 2011;12:391-406 PubMed

Exp Cell Res. 1972 Nov;75(1):304-6 PubMed

PLoS One. 2012;7(3):e34506 PubMed

Biol Lett. 2014 Mar 05;10(3):20131093 PubMed

Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 PubMed

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