The eyelid geckos (family Eublepharidae) include both species with temperature-dependent sex determination and species where genotypic sex determination (GSD) was suggested based on the observation of equal sex ratios at several incubation temperatures. In this study, we present data on karyotypes and chromosomal characteristics in 12 species (Aeluroscalabotes felinus, Coleonyx brevis, Coleonyx elegans, Coleonyx variegatus, Eublepharis angramainyu, Eublepharis macularius, Goniurosaurus araneus, Goniurosaurus lichtenfelderi, Goniurosaurus luii, Goniurosaurus splendens, Hemitheconyx caudicinctus, and Holodactylus africanus) covering all genera of the family, and search for the presence of heteromorphic sex chromosomes. Phylogenetic mapping of chromosomal changes showed a long evolutionary stasis of karyotypes with all acrocentric chromosomes followed by numerous chromosomal rearrangements in the ancestors of two lineages. We have found heteromorphic sex chromosomes in only one species, which suggests that sex chromosomes in most GSD species of the eyelid geckos are not morphologically differentiated. The sexual difference in karyotype was detected only in C. elegans which has a multiple sex chromosome system (X(1)X(2)Y). The metacentric Y chromosome evolved most likely via centric fusion of two acrocentric chromosomes involving loss of interstitial telomeric sequences. We conclude that the eyelid geckos exhibit diversity in sex determination ranging from the absence of any sexual differences to heteromorphic sex chromosomes, which makes them an interesting system for exploring the evolutionary origin of sexually dimorphic genomes.

Faculty of Science Charles University Prague Viničná 7 128 44 Praha 2 Czech Republic

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Chromosoma. 1976 Jan 27;54(1):75-87 PubMed

Chromosoma. 1995 May;103(9):642-52 PubMed

Neuroendocrinology. 2000 Apr;71(4):252-61 PubMed

Sex Dev. 2007;1(2):85-99 PubMed

Chromosoma. 2007 Apr;116(2):159-73 PubMed

Biol Lett. 2008 Apr 23;4(2):176-8 PubMed

Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):8147-52 PubMed

Genes Chromosomes Cancer. 1992 Apr;4(3):257-63 PubMed

J Exp Zool. 1993 May 1;265(6):679-83 PubMed

Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18190-5 PubMed

Exp Cell Res. 1972 Nov;75(1):304-6 PubMed

Genome Biol. 2007;8(11):R243 PubMed

Bioessays. 2004 Jun;26(6):639-45 PubMed

J Evol Biol. 2006 Nov;19(6):1775-84 PubMed

Am Nat. 2003 Apr;161(4):676-83 PubMed

Chromosome Res. 1999;7(6):461-74 PubMed

Naturwissenschaften. 2008 Mar;95(3):209-15 PubMed

Experientia. 1970;26(2):206-7 PubMed

Sex Dev. 2010;4(1-2):88-103 PubMed

Gene. 2008 Jan 15;407(1-2):105-15 PubMed

Chromosoma. 1982;86(2):149-79 PubMed

Gen Comp Endocrinol. 2005 Jul;142(3):289-96 PubMed

Cytogenet Genome Res. 2009;127(2-4):249-60 PubMed

Experientia. 1980 Aug 15;36(8):1014-5 PubMed

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