Here we present a new method to monitor fluoride transmembrane transport into liposomes using a europium(III) complex. We take advantage of the long emission lifetime of this probe to measure the transport activity of a fluorescent transporter. The high sensitivity, selectivity, and versatility of the assay allowed us to study different types of fluoride transporters and unravel their mechanisms of action.

Department of Chemistry and RECETOX Faculty of Science Masaryk University Brno 62500 Czech Republic

Department of Chemistry Loughborough University Epinal Way Loughborough UK

Department of Chemistry Texas A and M University College Station Texas 77843 USA

Université libre de Bruxelles Engineering of Molecular NanoSystems Avenue F D Roosevelt 50 CP165 64 B 1050 Brussels Belgium

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The F− transport experiment is performed at pH 7, at which the concentration of OH− is too low for the probe to sense

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