Attachment to a substrate to maintain position in a specific ecological niche is a common strategy across biology, especially for eukaryotic parasites. During development in the sand fly vector, the eukaryotic parasite Leishmania adheres to the stomodeal valve, as the specialised haptomonad form. Dissection of haptomonad adhesion is a critical step for understanding the complete life cycle of Leishmania. Nevertheless, haptomonad studies are limited, as this is a technically challenging life cycle form to investigate. Here, we have combined three-dimensional electron microscopy approaches, including serial block face scanning electron microscopy (SBFSEM) and serial tomography to dissect the organisation and architecture of haptomonads in the sand fly. We showed that the attachment plaque contains distinct structural elements. Using time-lapse light microscopy of in vitro haptomonad-like cells, we identified five stages of haptomonad-like cell differentiation, and showed that calcium is necessary for Leishmania adhesion to the surface in vitro. This study provides the structural and regulatory foundations of Leishmania adhesion, which are critical for a holistic understanding of the Leishmania life cycle.

Department of Basic Biology School of Life Science Okazaki Japan

Department of Biological and Medical Sciences Oxford Brookes University Oxford United Kingdom

Department of Parasitology Charles University Prague Czech Republic

Laboratory for Spatiotemporal Regulations National Institute for Basic Biology Okazaki Japan

Research Center of Mathematics for Social Creativity Research Institute for Electronic Science Hokkaido University Sapporo Japan

Spatiotemporal Regulations Group Exploratory Research Center for Life and Living Systems Okazaki Japan

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doi: 10.1101/2022.10.28.514187 PubMed

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Discovery of essential kinetoplastid-insect adhesion proteins and their function in Leishmania-sand fly interactions

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