Dicer structure and function: conserved and evolving features
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, přehledy
Grantová podpora
20-03950X
Czech Science Foundation
90140
Ministry of Education, Youth, and Sports of the Czech Republic
PubMed
37310138
PubMed Central
PMC10328071
DOI
10.15252/embr.202357215
Knihovny.cz E-zdroje
- Klíčová slova
- Dicer, dsRBD, helicase, miRNA, siRNA,
- MeSH
- dvouvláknová RNA genetika MeSH
- malá interferující RNA genetika metabolismus MeSH
- mikro RNA * genetika metabolismus MeSH
- ribonukleasa III * genetika MeSH
- RNA interference MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- dvouvláknová RNA MeSH
- malá interferující RNA MeSH
- mikro RNA * MeSH
- ribonukleasa III * MeSH
RNase III Dicer produces small RNAs guiding sequence-specific regulations, with important biological roles in eukaryotes. Major Dicer-dependent mechanisms are RNA interference (RNAi) and microRNA (miRNA) pathways, which employ distinct types of small RNAs. Small interfering RNAs (siRNAs) for RNAi are produced by Dicer from long double-stranded RNA (dsRNA) as a pool of different small RNAs. In contrast, miRNAs have specific sequences because they are precisely cleaved out from small hairpin precursors. Some Dicer homologs efficiently generate both, siRNAs and miRNAs, while others are adapted for biogenesis of one small RNA type. Here, we review the wealth of recent structural analyses of animal and plant Dicers, which have revealed how different domains and their adaptations contribute to substrate recognition and cleavage in different organisms and pathways. These data imply that siRNA generation was Dicer's ancestral role and that miRNA biogenesis relies on derived features. While the key element of functional divergence is a RIG-I-like helicase domain, Dicer-mediated small RNA biogenesis also documents the impressive functional versatility of the dsRNA-binding domain.
CEITEC Central European Institute of Technology Masaryk University Brno Czech Republic
Faculty of Science National Centre for Biomolecular Research Masaryk University Brno Czech Republic
Institute of Molecular Genetics of the Czech Academy of Sciences v v i Prague 4 Czech Republic
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