Whereas temporal variability of plant phenology in response to climate change has already been well studied, the spatial variability of phenology is not well understood. Given that phenological shifts may affect biotic interactions, there is a need to investigate how the variability in environmental factors relates to the spatial variability in herbaceous species' phenology by at the same time considering their functional traits to predict their general and species-specific responses to future climate change. In this project, we analysed phenology records of 148 herbaceous species, which were observed for a single year by the PhenObs network in 15 botanical gardens. For each species, we characterised the spatial variability in six different phenological stages across gardens. We used boosted regression trees to link these variabilities in phenology to the variability in environmental parameters (temperature, latitude and local habitat conditions) as well as species traits (seed mass, vegetative height, specific leaf area and temporal niche) hypothesised to be related to phenology variability. We found that spatial variability in the phenology of herbaceous species was mainly driven by the variability in temperature but also photoperiod was an important driving factor for some phenological stages. In addition, we found that early-flowering and less competitive species characterised by small specific leaf area and vegetative height were more variable in their phenology. Our findings contribute to the field of phenology by showing that besides temperature, photoperiod and functional traits are important to be included when spatial variability of herbaceous species is investigated.

Biodiversity Macroecology and Biogeography University of Goettingen Goettingen Germany

Biodiversity Research Institute IMIB Mieres Spain

Biology Department Boston University Boston MA USA

Botanic Garden Berlin Freie Universität Berlin Berlin Germany

Campus Institute Data Science University of Goettingen Goettingen Germany

Centre of Biodiversity and Sustainable Land Use University of Goettingen Goettingen Germany

Core Facility Botanical Garden University Vienna Vienna Austria

Department of Botany Faculty of Science Charles University Prague Czech Republic

Department of Botany University of Kashmir Srinagar Jammu and Kashmir India

Department of Ecosystem Services Helmholtz Centre for Environmental Research UFZ Leipzig Germany

Department of Environmental Biology Sapienza University of Rome Rome Italy

German Centre for Integrative Biodiversity Research Halle Jena Leipzig Leipzig Germany

Institute of Biochemistry and Biology Department of Biodiversity Research Systematic Botany with Botanical Garden University of Potsdam Potsdam Germany

Institute of Biodiversity Friedrich Schiller University Jena Jena Germany

Institute of Biology Geobotany and Botanical Garden Martin Luther University Halle Wittenberg Halle Germany

Institute of Ecology and Evolution with Herbarium Haussknecht and Botanical Garden Friedrich Schiller University Jena Jena Germany

Institute of Evolution and Ecology University of Tübingen Tübingen Germany

Institute of Plant Sciences Ecology and Conservation Biology University of Regensburg Regensburg Germany

Max Planck Institute for Biogeochemistry Jena Germany

Palmengarten and Botanical Garden Frankfurt Frankfurt am Main Germany

Petrozavodsk Republic of Karelia Russia

Ringve Botanical Garden NTNU University Museum Norwegian University of Science and Technology Trondheim Norway

Royal Botanic Garden Edinburgh Edinburgh UK

Systematic Botany and Functional Biodiversity Life Science Leipzig University Leipzig Germany

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