Role of the 3-mercaptopyruvate sulfurtransferase in colon/colorectal cancers
Language English Country Germany Media print-electronic
Document type Journal Article
PubMed
38631098
DOI
10.1016/j.ejcb.2024.151415
PII: S0171-9335(24)00032-3
Knihovny.cz E-resources
- Keywords
- 3- mercaptopyruvate sulfurtransferase, Colon/colorectal cancer, Fission, Mitochondria,
- MeSH
- Apoptosis MeSH
- Dynamins metabolism genetics MeSH
- HCT116 Cells MeSH
- Colorectal Neoplasms * metabolism pathology enzymology genetics MeSH
- Humans MeSH
- Membrane Potential, Mitochondrial MeSH
- Mitochondria metabolism MeSH
- Cell Line, Tumor MeSH
- Colonic Neoplasms metabolism pathology genetics enzymology MeSH
- Cell Movement MeSH
- Cell Proliferation MeSH
- Sulfurtransferases * metabolism genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- 3-mercaptopyruvate sulphurtransferase MeSH Browser
- DNM1L protein, human MeSH Browser
- Dynamins MeSH
- Sulfurtransferases * MeSH
The 3-mercaptopyruvate sulfurtransferase (MPST) is a protein persulfidase, occurring mainly in mitochondria. Although function of this protein in cancer cells has been already studied, no clear outcome can be postulated up to now. Therefore, we focused on the determination of function of MPST in colon (HCT116 cells)/colorectal (DLD1 cells) cancers. In silico analysis revealed that in gastrointestinal cancers, MPST together with its binding partners can be either of a high risk or might have a protective effect. Silencing of MPST gene resulted in decreased ATP, while acetyl-CoA levels were elevated. Increased apoptosis was detected in cells with silenced MPST gene, which was accompanied by decrease in mitochondrial membrane potential, but no changes in IP3 receptor's protein. Mitochondria underwent activation of fission and elevated DRP1 expression after MPST silencing. Proliferation and migration of DLD1 and HCT116 cells were markedly affected, showing the importance of MPST protein in colon/colorectal cancer development.
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