Methods for Evaluation of Antivirals and Neutralizing Antibodies Against Tick-Borne Encephalitis Virus
Language English Country United States Media print
Document type Journal Article
- Keywords
- Antivirals, Immunofluorescence, Monoclonal antibodies, Mouse infection, Neutralization test, Plaque assay, Tick-borne encephalitis virus,
- MeSH
- Antiviral Agents * pharmacology MeSH
- Cell Line MeSH
- Encephalitis, Tick-Borne * virology drug therapy immunology MeSH
- Humans MeSH
- Disease Models, Animal MeSH
- Antibodies, Monoclonal immunology MeSH
- Mice MeSH
- Neutralization Tests methods MeSH
- Antibodies, Neutralizing * immunology pharmacology MeSH
- Viral Plaque Assay MeSH
- Antibodies, Viral * immunology MeSH
- Encephalitis Viruses, Tick-Borne * drug effects immunology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antiviral Agents * MeSH
- Antibodies, Monoclonal MeSH
- Antibodies, Neutralizing * MeSH
- Antibodies, Viral * MeSH
Tick-borne encephalitis (TBE) is a potentially fatal neuroinfection of humans caused by the TBE virus. There is no specific therapy for TBE, as treatment is only supportive. Therefore, efforts to develop an effective specific therapy for TBE are warranted. Small molecule inhibitors and monoclonal antibodies are under intense investigation as potential therapeutics to combat TBE in humans. Here we describe the basic methods that can be used to test small molecule antivirals or monoclonal antibodies against TBEV. These include in vitro methods such as plaque assays, neutralizing assays, immunofluorescence staining of viral antigen, and cell-based antiviral assays, as well as in vivo assays in mouse model of TBE.
Faculty of Science Masaryk University Brno Czech Republic
Faculty of Veterinary Medicine University of Veterinary Sciences Brno Czech Republic
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