BACKGROUND: In patients with colorectal liver metastases, the possibility for radical liver resection can be limited by oxaliplatin-induced sinusoidal obstruction syndrome (SOS). This study investigates the potential of mesenchymal stem cells (MSC) to improve the outcome of liver resections in pigs with SOS. MATERIALS AND METHODS: SOS was induced in all animals (n=20) on day 0. Animals in the experimental group (n=8) received allogeneic MSC on day 7. Liver resection was performed in all animals on day 14 and the animals were observed until day 28. Ultrasound volumetry, biochemical analysis and histological examination of liver parenchyma was performed during the follow-up period. RESULTS: Six animals from the control group died prematurely, while all animals survived in the experimental group. According to histology, biochemical analysis and ultrasound volumetry, there were no significant differences between the groups documenting the effect of MSC. CONCLUSION: Single dose allogeneic MSC administration improved survival of animals with SOS undergoing partial liver resection. Further experiments with different timing of liver resection and MSC administration should be performed to investigate the effect of MSC in more detail.

• MeSH
• biologické markery MeSH
• hepatektomie * metody   MeSH
• imunofenotypizace MeSH
• imunohistochemie MeSH
• jaterní žilní okluze etiologie   patologie   terapie   MeSH
• kolorektální nádory patologie   MeSH
• kombinovaná terapie MeSH
• mezenchymální kmenové buňky * cytologie   MeSH
• modely nemocí na zvířatech MeSH
• nádory jater komplikace   sekundární   MeSH
• prasata MeSH
• transplantace mezenchymálních kmenových buněk * MeSH
• výsledek terapie MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND: In clinical medicine, little is known about the use of allografts for portal vein (PV) reconstruction after pancreaticoduodenectomy (PD). Portal and caval systems are physiologically different, therefore the properties of allografts from caval and portal systems were studied here in a pig model. MATERIALS AND METHODS: PD with PV reconstruction with allogeneic venous graft from PV or inferior vena cava (IVC) was performed in 26 pigs. Biochemical analysis and ultrasonography measurements were performed during a 4-week monitoring period. Computer simulations were used to evaluate haemodynamics in reconstructed PV and explanted allografts were histologically examined. RESULTS: The native PV and IVC grafts varied in histological structure but were able to adapt morphologically after transplantation. Computer simulation suggested PV grafts to be more susceptible to thrombosis development. Thrombosis of reconstructed PV occurred in four out of five cases in PV group. CONCLUSION: This study supports the use of allografts from caval system for PV reconstruction in clinical medicine when needed.

• MeSH
• alografty MeSH
• anastomóza chirurgická metody   MeSH
• hemodynamika MeSH
• léčba šetřící orgány MeSH
• mrtvola MeSH
• odběr tkání a orgánů MeSH
• pankreatoduodenektomie * MeSH
• počítačová simulace * MeSH
• pooperační komplikace etiologie   MeSH
• prasata MeSH
• pylorus MeSH
• regionální krevní průtok MeSH
• ultrasonografie MeSH
• velikost orgánu MeSH
• vena cava inferior anatomie a histologie   diagnostické zobrazování   fyziologie   transplantace   MeSH
• vena portae anatomie a histologie   diagnostické zobrazování   fyziologie   chirurgie   MeSH
• zákroky plastické chirurgie metody   MeSH
• žilní trombóza etiologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH