BACKGROUND: Interaction of CD200 with its receptor CD200R has an immunoregulatory role and attenuates various types of neuroinflammatory diseases. METHODS: Immunofluorescence staining, western blot analysis, and RT-PCR were used to investigate the modulatory effects of CD200 fusion protein (CD200Fc) on activation of microglia and astrocytes as well as synthesis of pro- (TNF, IL-1β, IL-6) and anti-inflammatory (IL-4, IL-10) cytokines in the L4-L5 spinal cord segments in relation to behavioral signs of neuropathic pain after unilateral sterile chronic constriction injury (sCCI) of the sciatic nerve. Withdrawal thresholds for mechanical hypersensitivity and latencies for thermal hypersensitivity were measured in hind paws 1 day before operation; 1, 3, and 7 days after sCCI operation; and then 5 and 24 h after intrathecal application of artificial cerebrospinal fluid or CD200Fc. RESULTS: Seven days from sCCI operation and 5 h from intrathecal application, CD200Fc reduced mechanical and thermal hypersensitivity when compared with control animals. Simultaneously, CD200Fc attenuated activation of glial cells and decreased proinflammatory and increased anti-inflammatory cytokine messenger RNA (mRNA) levels. Administration of CD200Fc also diminished elevation of CD200 and CD200R proteins as a concomitant reaction of the modulatory system to increased neuroinflammatory reactions after nerve injury. The anti-inflammatory effect of CD200Fc dropped at 24 h after intrathecal application. CONCLUSIONS: Intrathecal administration of the CD200R1 agonist CD200Fc induces very rapid suppression of neuroinflammatory reactions associated with glial activation and neuropathic pain development. This may constitute a promising and novel therapeutic approach for the treatment of neuropathic pain.

• MeSH
• antigeny povrchové farmakologie   terapeutické užití   MeSH
• časové faktory MeSH
• CD antigeny metabolismus   MeSH
• cytokiny genetika   metabolismus   MeSH
• fyzikální stimulace škodlivé účinky   MeSH
• gliový fibrilární kyselý protein metabolismus   MeSH
• hyperalgezie farmakoterapie   etiologie   MeSH
• ischialgie komplikace   farmakoterapie   MeSH
• krysa rodu rattus MeSH
• mícha účinky léků   metabolismus   MeSH
• modely nemocí na zvířatech MeSH
• neuroglie účinky léků   metabolismus   MeSH
• potkani Wistar MeSH
• práh bolesti účinky léků   MeSH
• receptory buněčného povrchu antagonisté a inhibitory   terapeutické užití   MeSH
• regulace genové exprese účinky léků   MeSH
• spinální injekce MeSH
• zánět farmakoterapie   etiologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH