Dysregulated iron metabolism has a detrimental effect on cardiac function. The importance of iron homeostasis in cardiac health and disease warrants detailed studies of cardiomyocyte iron uptake, utilization and recycling at the molecular level. In this study, we have performed metabolic labeling of primary cultures of neonatal rat cardiomyocytes with radioactive iron coupled with separation of labeled iron-containing molecules by native electrophoresis followed by detection and quantification of incorporated radioiron by storage phosphorimaging. For the radiolabeling we used a safe and convenient beta emitter 55Fe which enabled sensitive and simultaneous detection and quantitation of iron in cardiomyocyte ferritin, transferrin and the labile iron pool (LIP). The LIP is believed to represent potentially dangerous redox-active iron bound to uncharacterized molecules. Using size-exclusion chromatography spin micro columns, we demonstrate that iron in the LIP is bound to high molecular weight molecule(s) (≥5000 Da) in the neonatal cardiomyocytes.

• MeSH
• biologický transport MeSH
• chelátory chemie   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• ferritin chemie   metabolismus   MeSH
• gelová chromatografie MeSH
• homeostáza MeSH
• kardiomyocyty metabolismus   MeSH
• kultivované buňky MeSH
• limita detekce MeSH
• potkani Wistar MeSH
• radioizotopy železa metabolismus   MeSH
• transferin chemie   metabolismus   MeSH
• železo chemie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH