Disturbance of capillary perfusions due to leukocyte adhesion, disseminated intravascular coagulation, tissue edema is critical components in the pathophysiology of sepsis. Alterations in brain microcirculation during sepsis are not clearly understood. The aim of this study is to gain an improved understanding of alterations through direct visualization of brain microcirculations in an experimental endotoxemia using intravital microscopy (IVM). Endotoxemia was induced in Lewis rats with Lipopolysaccharide (LPS, 15 mg/kg i.v.). The dura mater was removed via a cranial window to expose the pial vessels on the brain surface. Using fluorescence dyes, plasma extravasation of pial venous vessels and leukocyte-endothelial interaction were visualized by intravital microscopy 4 h after LPS administration. Plasma cytokine levels of IL1-β, IL-6, IFN-γ, TNF-α and KC/GRO were evaluated after IVM. A significant plasma extravasation of the pial venous vessels was found in endotoxemia rats compared to control animals. In addition, a significantly increased number of leukocytes adherent to the pial venous endothelium was observed in septic animals. Endotoxemia also induced a significant elevation of plasma cytokine levels of IL1-β, IL-6, IFN-γ, TNF-α and KC/GRO. Endotoxemia increased permeability in the brain pial vessels accompanied by an increase of leukocyte-endothelium interactions and an increase of inflammatory cytokines in the plasma.

• MeSH
• buněčná adheze MeSH
• cévní endotel metabolismus   patofyziologie   MeSH
• endotoxemie metabolismus   patofyziologie   MeSH
• interferon gama krev   MeSH
• interleukin-1beta krev   MeSH
• interleukin-6 krev   MeSH
• kapilární permeabilita MeSH
• krysa rodu rattus MeSH
• leukocyty fyziologie   MeSH
• mikrocévy metabolismus   patofyziologie   MeSH
• mikrocirkulace fyziologie   MeSH
• mozek krevní zásobení   MeSH
• mozkové žíly metabolismus   MeSH
• potkani inbrední LEW MeSH
• TNF-alfa krev   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH