Derivatization of amino acids by using ethyl chloroformate-ethanol-pyridine provides volatile products, N-ethoxycarbonyl amino acid ethyl esters (ECEEs), which are easily amenable to GC or GC-MS analysis. MS behavior of these compounds under electron-impact has been studied. The fragments observed in the spectra facilitate recognition of commonly occurring protein amino acids and characterization of unknown analogues.

• MeSH
• aminokyseliny * chemie   MeSH
• estery chemie   MeSH
• hmotnostní spektrometrie MeSH
• Publikační typ
• Research Support, U.S. Gov't, P.H.S. MeSH