The luxS gene is responsible for the synthesis of AI-2 (autoinducer-2), a signaling molecule that participates in quorum sensing regulation in a large number of bacteria. In this work, we investigated which phenotypes are regulated by luxS gene in Serratia proteamaculans 94, psychrotrophic strain isolated from spoiled refrigerated meat. AI-2 was identified in S. proteamaculans 94, and the luxS gene involved in its synthesis was cloned and sequenced. A mutant with the inactivated luxS gene was constructed. Inactivation of the luxS gene was shown to lead to the absence of AI-2 synthesis, chitinolytic activity, swimming motility, suppression of the growth of fungal plant pathogens Rhizoctonia solani and Helminthosporium sativum by volatile compounds emitted by S. proteamaculans 94 strain, and to a decrease of extracellular proteolytic activity. The knockout of the luxS gene did not affect synthesis of N-acyl-homoserine lactones, lipolytic, and hemolytic activities of S. proteamaculans 94.
- MeSH
- bakteriální proteiny genetika MeSH
- biofilmy růst a vývoj MeSH
- fenotyp MeSH
- homoserin analogy a deriváty metabolismus MeSH
- laktony metabolismus MeSH
- lyasy štěpící vazby C-S genetika MeSH
- maso mikrobiologie MeSH
- mikrobiální interakce MeSH
- quorum sensing genetika MeSH
- regulace genové exprese u bakterií MeSH
- Serratia genetika metabolismus MeSH
- těkavé organické sloučeniny analýza MeSH
- umlčování genů * MeSH
- Publikační typ
- časopisecké články MeSH
