Goeckerman therapy (GT) for psoriasis combines the therapeutic effect of crude coal tar (CCT) and ultraviolet radiation (UVR). CCT contains polycyclic aromatic hydrocarbons, some of which can form DNA adducts that may induce mutations and contribute to carcinogenesis. The aim of our work was to evaluate the relationship between concentrations of benzo[a]pyrene-7,8-diol-9,10-epoxide-DNA adducts (BPDE-DNA adducts) and rs4646903 (CYP1A1 gene), rs1048943 (CYP1A1), rs1056836 (CYP1B1), rs1051740 (EPHX1), rs2234922 (EPHX1) and rs8175347 (UGT1A1) polymorphic sites, and GSTM1 null polymorphism in 46 patients with chronic stable plaque psoriasis who underwent GT. The level of BPDE-DNA adducts was determined using the OxiSelect BPDE-DNA Adduct ELISA Kit. Polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (rs4646903, rs1048943, rs1051740, and rs2234922), fragment analysis (rs8175347), real-time PCR (rs1056836), and digital droplet PCR polymorphism (GSTM1) were used. CYP1B1*1/*1 wild-type subjects and CYP1B1*3/*1 heterozygotes for rs1056836 formed significantly higher amounts of BPDE-DNA adducts than CYP1B1*3/*3 homozygotes (p=0.031 and p=0.005, respectively). Regarding rs1051740, individuals with EPHX1*3/*1 heterozygosity revealed fewer adducts than EPHX1*1/*1 wild-type subjects (p=0.026). Our data suggest that CYP1B1/EPHX1 genotyping could help to predict the risk of DNA damage and to optimize doses of coal tar and UVR exposure in psoriatic patients in whom GT was applied.
- MeSH
- 7,8-dihydro-7,8-dihydroxybenzo(a)pyren 9,10-oxid metabolismus MeSH
- adukty DNA metabolismus MeSH
- aplikace kožní MeSH
- benzopyren aplikace a dávkování škodlivé účinky metabolismus MeSH
- biotransformace MeSH
- cytochrom P450 CYP1B1 genetika metabolismus MeSH
- dehet uhelný aplikace a dávkování škodlivé účinky metabolismus MeSH
- dospělí MeSH
- epoxid hydrolasy genetika metabolismus MeSH
- farmakogenetika MeSH
- fenotyp MeSH
- frekvence genu MeSH
- glukuronosyltransferasa genetika metabolismus MeSH
- glutathiontransferasa genetika metabolismus MeSH
- heterozygot MeSH
- hodnocení rizik MeSH
- homozygot MeSH
- keratolytika aplikace a dávkování škodlivé účinky metabolismus MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- polymorfismus genetický * MeSH
- poškození DNA MeSH
- psoriáza enzymologie genetika terapie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- terapie ultrafialovými paprsky * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Some specific antibodies indicate the presence of antigenic structures on DNA (DNA adducts) that can play an important role in the process of mutagenesis and/or carcinogenesis. They indicate the presence of increased genotoxic potential (hazard) prior to the formation of disease (primary prevention). The present study was focused on the serum level of benzo[a]pyrene 7,8-diol-9,10-epoxide-DNA adducts antibodies (anti-BPDE-DNA) in psoriatic patients (n = 55) dermally exposed to different levels of polycyclic aromatic hydrocarbons (PAHs). The general goal of the study was to contribute to better understanding of the value of the assumed biomarker (anti-BPDE-DNA) for evaluation of the organism's answer to genotoxic exposure to PAHs. Elevated level of exposure to PAHs resulted in the increased level of anti-BPDE-DNA. However, almost all levels of anti-BPDE-DNA ranged within the field of low values. Both variants of GT (CCT-3% and CCT-5%) induced higher expression of anti-BPDE-DNA in the group of nonsmokers. Significant relations between the level of anti-BPDE-DNA and PASI score, total duration of the therapy, or time of UVR exposure were not found. Further studies are needed to reduce interpretation uncertainty of this promising bioindicator.
- MeSH
- 7,8-dihydro-7,8-dihydroxybenzo(a)pyren 9,10-oxid MeSH
- adukty DNA krev imunologie MeSH
- biologické markery krev MeSH
- dehet uhelný terapeutické užití MeSH
- dospělí MeSH
- kouření krev MeSH
- kůže účinky léků imunologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- polycyklické aromatické uhlovodíky toxicita MeSH
- protilátky krev MeSH
- psoriáza krev imunologie patologie terapie MeSH
- senioři MeSH
- terapie ultrafialovými paprsky MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
