Aortal valve mineralization very frequently causes a genesis of aortic stenosis, which is the most often surgically treated heart disease. Hydroxyapatite deposits have been identified as one of the causes leading to the loss of elasticity of the aortic valves. It is known that phosphates/calcium is accumulated in valve tissues during mineralization, but the mechanism of this process remains unclear. The work is focused mainly on the study of protein composition of mineralized aortic valves by nano-liquid chromatography electrospray ionization in a quadrupole orthogonal acceleration time-of-flight mass spectrometry. New methodological approach based on direct enzymatic digestion of proteins contained in hydroxyapatite deposits was developed for the study of pathological processes connected with osteogenesis. Our objectives were to simplify the traditional analytical protocols of sample preparation and to analyze the organic components of the explanted aortic valves for significant degenerative aortic stenosis. The study of aortic valve mineralization on the molecular level should contribute to understanding this process, which should consequently lead to effective prevention as well as to new ways of treatment of this grave disease.

• MeSH
• aorta chemie   patologie   MeSH
• aortální chlopeň chemie   patologie   MeSH
• aortální stenóza metabolismus   patologie   MeSH
• biopsie MeSH
• hmotnostní spektrometrie s elektrosprejovou ionizací MeSH
• hydroxyapatit chemie   MeSH
• kalcinóza patologie   MeSH
• lidé MeSH
• peptidové fragmenty analýza   MeSH
• proteiny chemie   izolace a purifikace   MeSH
• proteolýza MeSH
• trypsin chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Aortic stenosis is one of the most common heart diseases that occur in developed countries. The disease has many causes; among the most discussed is excessive intake of fluorides. The aim of our work is to clarify the biochemical nature of biomineralizations in cardial tissue as well as to select an appropriate medication and to mitigate the disease. Peptide mapping combined with UHPLC and MS were used in the study of the mineralized cardial tissue. We managed to identify proteins such as alkaline phosphatase, biglycan, mimecan, osteopontin, periostin and proteoglycan, which are probably related to mineralization of the valves.

• MeSH
• aortální stenóza * etiologie   MeSH
• chromatografie kapalinová využití   MeSH
• fluor škodlivé účinky   MeSH
• hmotnostní spektrometrie * metody   přístrojové vybavení   využití   MeSH
• kalcinóza * patofyziologie   MeSH
• lidé MeSH
• peptidové mapování * metody   trendy   využití   MeSH
• tandemová hmotnostní spektrometrie využití   MeSH
• vitamin K chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH