An integral part of the research and development of new antidotes against nerve agents (herein referred to as NAs) is the verification of their efficacy by means of in vitro tests. The purpose of these tests is to verify the ability of an antidote to protect cholinesterases from inhibition or to reactivate inhibited cholinesterases. For this purpose cholinesterases of different species and with a different degree of purification are traditionally used. Using immobilized cholinesterases proves to be new and advantageous for a number of reasons. The authors of the presented work verified the usability of Detehit detector (Oritest Praha Ltd) containing the pig’s brain acetylcholinesterase for this purpose. In this article we see the results of tests focused on verifying the effect of different reaction conditions on the activity of acetylcholinesterase, especially with regard to reproducibility of these measurements.

• MeSH
• acetylcholinesterasa účinky léků   MeSH
• antidota terapeutické užití   MeSH
• biosenzitivní techniky metody   využití   MeSH
• buněčné kultury využití   MeSH
• chemické bojové látky škodlivé účinky   toxicita   MeSH
• mozek enzymologie   MeSH
• reaktivátory cholinesterasy terapeutické užití   MeSH
• Sus scrofa MeSH
• techniky in vitro MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH