Molecular diversity of rumen bacteria belonging to the species Selenomonas ruminantium was evaluated by biochemical and PCR analyses targeted at the 16S rRNA operon and lactate dehydrogenase gene. While extremely variable in metabolic characteristics, two different RISA (ribosomal intergenic spacer analysis), and five lactate dehydrogenase gene RFLP profiles were observed among the twelve strains studied. The strains showed very limited variability ARDRA ( amplified ribosomal DNA restriction analysis) when two different profiles were observed only. 16S rDNA sequence comparisons indicate complex genetic structure within S.ruminantium population.
- MeSH
- bachor mikrobiologie MeSH
- bakteriální proteiny genetika MeSH
- DNA bakterií genetika MeSH
- fylogeneze MeSH
- genetická variace MeSH
- L-laktátdehydrogenasa genetika MeSH
- molekulární sekvence - údaje MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- ribozomální DNA genetika MeSH
- RNA ribozomální 16S genetika MeSH
- techniky typizace bakterií MeSH
- Veillonellaceae genetika izolace a purifikace klasifikace MeSH
- vysoká zvěř mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
Molecular analysis of isolates of the rumen bacterium Selenomonas ruminantium revealed a high variety and frequency of site-specific (restriction) endonucleases. While all known S. ruminantium restriction and modification systems recognize hexanucleotide sequences only, consistently low counts of both 6-bp and 4-bp palindromes were found in DNA sequences of S. ruminantium. Statistical analysis indicated that there is some correlation between the degree of underrepresentation of tetranucleotide words and the number of known restriction endonucleases for a given sequence. Control analysis showed the same correlation in lambda DNA but not in human adenovirus DNA. Based on the data presented, it could be proposed that there is a much higher historical occurrence of restriction and modification systems in S. ruminantium and (or) frequent horizontal gene transfer of restriction and modification gene complexes.
