Oocyte meiotic maturation and embryogenesis are some of the most important physiological processes that occur in organisms, playing crucial roles in the preservation of life in all species. The post-transcriptional regulation of maternal messenger ribonucleic acids (mRNAs) and the post-translational regulation of proteins are critical in the control of oocyte maturation and early embryogenesis. Translational control affects the basic mechanism of protein synthesis, thus, knowledge of the key components included in this machinery is required in order to understand its regulation. Cytoplasmic polyadenylation element binding proteins (CPEBs) bind to the 3'-end of mRNAs to regulate their localization and translation and are necessary for proper development. In this study we examined the expression pattern of cytoplasmic polyadenylation element binding protein 2 (CPEB2) both on the mRNA (by real-time quantitative reverse transcription polymerase chain reaction, qRT-PCR) and protein (by Western blotting, WB) level, as well as its localization during the meiotic maturation of porcine oocytes and early embryonic development by immunocytochemistry (ICC). For the elucidation of its functions, CPEB2 knockdown by double-strand RNA (dsRNA) was used. We discovered that CPEB2 is expressed during all stages of porcine meiotic maturation and embryonic development. Moreover, we found that it is necessary to enable a high percentage of oocytes to reach the metaphase II (MII) stage, as well as for the production of good-quality parthenogenetic blastocysts.
- MeSH
- 3' Untranslated Regions MeSH
- Embryonic Development MeSH
- Gene Knockdown Techniques MeSH
- Meiosis * MeSH
- RNA, Messenger metabolism MeSH
- Oocytes cytology metabolism MeSH
- Parthenogenesis MeSH
- Swine MeSH
- RNA-Binding Proteins genetics metabolism MeSH
- Pregnancy MeSH
- Gene Expression Regulation, Developmental MeSH
- Animals MeSH
- Check Tag
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Cílem práce bylo zjistit rozsah maximálního otevření úst (abdukce) u pacientů před ortognátní operací a v intervalu 2,3,4 a 9 měsíců po ní. Dále bylo cílem posoudit, zda je rozsah abdukce závislý na pohlaví, původní skeletální vadě nebo typu provedené operace. v naší retrospektivní studii jsme zjistili, že 2 měsíce po ortognátní operaci klesl rozsah abdukce průměrně na 56 % jejího rozsahu před operací, 3 měsíce po operaci se zvýšil na 68,8 %, po 4 měsících na 74 % a po 9 měsících dosáhl rozsah otevírání úst 91,1% původní hodnoty. Ve všech intervalech dosáhl rozsah otevírání úst u mužů vyšších hodnot než u žen a u pacientů s původní lil. skeletální třídou vyšších hodnot než u pacientů s původní II. skeletální třídou. Pacienti po operaci typu Le Fort I měli výrazně lepšíabdukci mandibuly ve 2., 3. a 4. měsíci po operaci, 9 měsíců po operaci již rozdíl nebyl statisticky ani klinicky významný. Nebyl nalezen klinicky ani statisticky významný rozdíl v abdukci ve všech sledovaných inten/alech mezi pacienty s BSSO a pacienty s bimaxilární operaci. Ani po devíti měsících od operace nedošlo u všech pacientů k obnovení původní pohyblivosti dolní čelisti.
The aim of the presented study was to measure maximum mandibular range of motion (abduction) prior to orthognathic surgery, and within the intervals of 2, 3, 4 and 9 months after orthognathic surgery. Another aim was to determine whether the range of abduction depends on gender, original skeletal deviation, and the kind of surgery. Our retrospect/ve study revealed that 2 months after orthognathic surgery the range of abduction decreased to 56 % of the abduction priorto surgery; 3 months after orthognathic surgery it raised to 68.8 %, after 4 months to 74 %, and after 9 months to 91.1 % of the original range. At all intervals, the range of motion was larger in male patients than in females, as well as in patients with the original skeletal Class III than in patients with Class II. The patients who undenivent LeFort I surgery showed significantly better mandibular motion 2,3, and 9 months after the surgery. After 9 months the difference was no longer statistically and clinically significant. There was found no clinically or statistically significant difference in mouth opening at all intervals between patients with BSSO and patients with bimaxillary surgery. Nine months after the surgery the original mandibular range of motion was not achieved in all patients.
- Keywords
- rozsah otevírání úst,
- MeSH
- Adult MeSH
- Physical Examination methods MeSH
- Middle Aged MeSH
- Humans MeSH
- Mandible MeSH
- Young Adult MeSH
- Orthognathic Surgical Procedures * MeSH
- Postoperative Complications MeSH
- Postoperative Period MeSH
- Preoperative Period MeSH
- Surgical Clearance MeSH
- Retrospective Studies MeSH
- Range of Motion, Articular * MeSH
- Temporomandibular Joint MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Young Adult MeSH
- Male MeSH
- Female MeSH
