The family of human matrix metalloproteinases (MMPs) comprises several tightly regulated classes of proteases. These enzymes and their specific inhibitors play important roles in tumour progression and the metastatic process by facilitating extracellular matrix degradation. As scientific understanding of the MMPs has advanced, therapeutic strategies focusing on blocking these enzymes by matrix metalloproteinase inhibitors have rapidly developed. Low molecular weight tissue inhibitors of matrix metalloproteinase (TIMPs) represent a new therapeutic approach for the treatment of individual types of cancer. This paper aims to briefly summarize current knowledge about the role of MMPs in select non- tumorous lesions, tumor invasion and metastasis. The perspectives in therapeutic intervention in cancer are also mentioned. The role of MMPs in diagnosis and prognosis of colorectal and thyroid cancer is discussed in detail.
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- financování organizované MeSH
- inhibitory matrixových metaloproteinas MeSH
- inhibitory proteas terapeutické užití MeSH
- lidé MeSH
- matrixové metaloproteinasy MeSH
- nádory štítné žlázy enzymologie farmakoterapie MeSH
- nádory enzymologie farmakoterapie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Aims: The diff erential diagnosis of thyroid nodules in routine practice can be problemmatic for both pathologistsand clinicians. Eff ective treatment requires a determination of the biological nature of the lesions. For this reason, ancilliarydiagnostic markers along with histological examination of the nodules may be useful. The objective of this studywas to evaluate the diagnostic usefulness of novel markers in the diagnosis of hyperplastic and neoplastic nodules.Methods: Forty eight thyroid lesions forming four diagnostic groups including adenomatous goiters (AS), follicularadenomas (FA), follicular (FC) and papillary carcinomas (PC) were examined using standard immunohistochemicalmethods. Monoclonal antibodies against galectin-3, matrix metalloproteinases (MMPs) -2 and -7 and endothelialmarkers CD31 and CD105 were used.Results: The cytoplasmatic expression of galectin-3 was positive in all cases of papillary carcinoma. Moreover,statistically signifi cant diff erences between fused groups of benign (AS and FA) and malignant lesions (FC and PC)were found Fischer’s exact test (p = 0.0001). No signifi cant diff erences in cytoplasmic expression of MMPs -2 and -7and in vascular density assessed by using of both endothelial markers between benign lesions and malignant tumorswere revealed.Conclusions: Galectin-3 appears to be a useful marker in the diagnosis of papillary carcinoma only. The matrixmetalloproteinases-2 and -7 are not helpful in distinguishing hyperplastic and neoplastic thyroid nodules. Endothelialmarkers do not appear to be suitable for thyroid diff erential diagnosis. A panel of antibodies in the diff erential diagnosisof thyroid nodular lesions would seem most suitable and further studies with larger sets of patients are awaited.
- MeSH
- antigeny CD31 izolace a purifikace MeSH
- diferenciální diagnóza MeSH
- folikulární adenokarcinom diagnóza etiologie MeSH
- galektin 3 izolace a purifikace MeSH
- hyperplazie diagnóza etiologie MeSH
- imunohistochemie metody využití MeSH
- látky indukující angiogenezi izolace a purifikace MeSH
- lidé MeSH
- matrixové metaloproteinasy izolace a purifikace MeSH
- monoklonální protilátky diagnostické užití MeSH
- nádorové biomarkery izolace a purifikace MeSH
- nádory štítné žlázy diagnóza etiologie MeSH
- papilární adenokarcinom diagnóza etiologie MeSH
- receptory buněčného povrchu izolace a purifikace MeSH
- statistika jako téma MeSH
- uzly štítné žlázy diagnóza etiologie MeSH
- Check Tag
- lidé MeSH
