Carbapenemase-producing Gram-negative bacteria peak clinical interest due to their ability to hydrolyze most β-lactams, including carbapenems; moreover, their genes spread through bacterial populations by horizontal transfer. Bacteria with acquired carbapenemase have sporadically been reported in the Czech Republic, so far only in Enterobacteriaceae and Pseudomonas aeruginosa. In this study, we described the first finding of a KPC-2-producing strain of Klebsiella pneumoniae, which was isolated from a surgical wound swab, decubitus ulcer, and urine of a patient previously hospitalized in Greece. The patient underwent various antibiotic therapies including a colistin treatment. However, after approximately 20 days of the colistin therapy, the strain developed a high-level resistance to this drug. All the isolates were indistinguishable by pulsed field gel electrophoretic analysis and belonged to the international clone ST258, which is typical of KPC-producing K. pneumoniae isolates. The bla (KPC-2) gene was located on a Tn4401a transposon variant. The OmpK35 and OmpK36 genes analysis performed due to the high resistance level of the strains to β-lactams exhibited no changes in their sequence or in their expression when compared with carbapenem-susceptible isolates.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální léková rezistence účinky léků genetika MeSH
- beta-laktamasy biosyntéza genetika MeSH
- hospitalizace MeSH
- Klebsiella pneumoniae účinky léků enzymologie genetika izolace a purifikace MeSH
- kolistin farmakologie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- poriny genetika MeSH
- regulace genové exprese u bakterií MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Řecko MeSH
Získané ß-laktamázy AmpC poedstavují vážný klinický a epidemiologický problém. V České republice (ČR) byly dosud popsány pouze u druhu Klebsiella pneumoniae a Serratia marcescens (DHA-1). Elánek popisuje záchyt dvou kmenu druhu Proteus mirabilis produkujících ß-laktamázy CMY-2. Jedná se o první doložený poípad produkce získané AmpC odlišné skupiny než je DHA-1 v ČR u humánních vzorku.
Acquired AmpC ß-lactamases are an important clinical and epidemiological problem. In the Czech Republic, they have only been detected in Klebsiella pneumoniae and Serratia marcescens (DHA-1). This article reports the detection of two CMY-2-producing strains of Proteus mirabilis. It is the first report of acquired AmpCblactamase other than DHA-1 in human isolates from the Czech Republic.
