During the period 2000-2003, patients hospitalized for suspected tick-borne encephalitis in the Czech Republic were screened for possible A. phagocytophilum co-infection. Blood samples taken at admission were tested for the presence of A. phagocytophilum DNA by nested PCR using a modified target sequence as an internal control, and sera were tested for the presence of antibodies by indirect immunofluorescence and western blotting methods using cell-culture-derived antigens. To verify the assay specificity, a set of 45 sera of Patagonian residents served as a non-tick-exposed control group, and a set of 14 B. henselae-positive sera was used to check cross-reactivity. Of 809 patients hospitalized, 80 (9.9%) showed IgG antibodies reactive to A. phagocytophilum at > or =80 (reciprocal dilution factor) and 50 (6.2%) at > or =160; two (0.2%) patients showed elevated IgM titers of 40. No full blood obtained from 162 patients tested positive in PCR when false negativity was excluded. During hospitalization, the diagnosis of tick-borne encephalitis was confirmed in 536 patients, 57 (10.6%) of whom had anti-A. phagocytophilum IgG antibodies reactive at > or =80 and 41 (7.6%) at > or =160, which did not differ significantly from the whole set (P = 0.66/0.30), the maximum IgG titer registered was 5120, and no IgM titer reached the 40 cut-off. Available paired sera from 189 tick-borne encephalitis patients showed no significant shifts, but one case of slight seroconversion (IgG shift from < 80 to 320) was detected in one of the non-tick-borne encephalitis patients. The sex of the patient showed no significance for the prevalence of A. phagocytophilum antibodies; however, the seropositive patients were older on average than those who were seronegative (43.5 +/- 15.9 vs. 37.9 +/- 18.3 years, P = 0.05). Clinical manifestation of the disease did not differ noticeably between patients with and without A. phagocytophilumreactive antibodies, except for fever duration, which was significantly longer in patients with titers > or =1280. Overall, A. phagocytophilum co-infection did not seem to be a frequent and/or significant complication of tick-borne encephalitis acquired in the Czech Republic.
- MeSH
- Anaplasma phagocytophilum genetika MeSH
- dítě MeSH
- DNA bakterií analýza MeSH
- dospělí MeSH
- ehrlichióza MeSH
- fluorescenční protilátková technika nepřímá MeSH
- imunoglobulin G imunologie krev MeSH
- klíšťová encefalitida MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- polymerázová řetězová reakce MeSH
- předškolní dítě MeSH
- protilátky bakteriální imunologie krev MeSH
- senioři MeSH
- studie případů a kontrol MeSH
- věkové faktory MeSH
- western blotting MeSH
- zkřížené reakce MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- předškolní dítě MeSH
- senioři MeSH
- Geografické názvy
- Česká republika MeSH
Idiopatická trombocytopenická purpura (ITP) je nejčastější příčinou akutní trombocytopénie, charakterizované krvácivými projevy. V etiologii tohoto onemocnění hrají významnou úlohu různá infekční agens. Popsány jsou 2 případy akutní ITP u pacientů hospitalizovaných na infekčním oddělení NsP Příbram pro salmonelózu a varicelu.
Idiopathic thrombocytopenic purpura (ITP) is the most frequent cause of acute thrombocytopenia with characteristic haemarrhagic manifestations. Different infectious agents play a significant role in the aetiology of this disorder. The authors describe two cases of acute ITP in patients admitted for saimoneiosis and varicella to the Dpt. of infectious diseases of the hospital in Příbram.
- MeSH
- lidé MeSH
- plané neštovice komplikace MeSH
- salmonelóza komplikace MeSH
- trombocytopenická purpura diagnóza etiologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- kazuistiky MeSH
