- Publikační typ
- abstrakt z konference MeSH
It is well known that antagonists of N-type voltage-gated calcium channels inhibit the evoked quantal release of acetylcholine in amphibian neuromuscular synapses. This, however, does not exclude the functional expression of other types of voltage-gated calcium channels in these nerve terminals. Using immunocytochemistry, we detected the expression of the α1A subunit of P/Q-type calcium channels (that is otherwise typical of mammalian motor nerve endings) in the frog neuromuscular junction. In addition, we demonstrated that the P/Q-type channel blocker ω-agatoxin IVA (20 nM) reduced the action potentialinduced calcium transient and significantly decreased both spontaneous and evoked mediator release. Our data indicates the functional expression of P/Q-type calcium channels in the frog motor nerve ending which participate in acetylcholine release.
- MeSH
- gating iontového kanálu fyziologie MeSH
- membránové potenciály fyziologie MeSH
- nervosvalové spojení fyziologie MeSH
- nervový přenos fyziologie MeSH
- Rana ridibunda MeSH
- vápník metabolismus MeSH
- vápníková signalizace fyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
A novel derivative of 6-methyluracil, C-547, increased the amplitude and prolonged the duration of miniature endplate currents (MEPCs) which is typical for acetylcholinesterase inhibition. In the soleus and extensor digitorum longus significant potentiation was detected at nanomolar concentrations. In contrast, in the diaphragm muscle, the increase in the amplitudes of the MEPCs and the decay time constant ? appeared only when the concentration of C-547 was elevated to 1x10-7 M. Possible consequences for the exploitation of this drug, which can selectively inhibit AChE in particular synapses, are discussed.
- MeSH
- acetylcholinesterasa farmakokinetika účinky léků MeSH
- bránice fyziologie účinky léků MeSH
- cholinesterasové inhibitory farmakokinetika chemie MeSH
- elektrofyziologie metody statistika a číselné údaje MeSH
- financování vládou MeSH
- interpretace statistických dat MeSH
- nervosvalová ploténka enzymologie fyziologie chemie MeSH
- potkani Wistar fyziologie MeSH
- svaly fyziologie účinky léků MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- acetylcholin analogy a deriváty farmakologie MeSH
- chemická deprese MeSH
- draslík metabolismus MeSH
- nervosvalová ploténka účinky léků MeSH
- nervosvalové spojení účinky léků MeSH
- parasympatomimetika farmakologie MeSH
- techniky in vitro MeSH
- vápník metabolismus MeSH
- žáby MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
