BACKGROUND: Several new species of Leishmania have recently emerged in Europe, probably as the result of global changes and increased human migration from endemic areas. In this study, we tested whether two sand fly species, the Western Mediterranean Phlebotomus perniciosus and the Eastern Mediterranean P. tobbi, are competent vectors of L. donovani, L. major and L. martiniquensis. METHODOLOGY/PRINCIPAL FINDINGS: Sand flies were infected through the chick skin membrane using Leishmania species and strains of various geographical origins. Leishmania infections were evaluated by light microscopy and qPCR, and the representation of morphological forms was assessed from Giemsa-stained gut smears. Neither P. perniciosus nor P. tobbi supported the development of L. martiniquensis, but L. major and L. donovani in both species survived defecation of blood meal remnants, colonized the stomodeal valve and produced metacyclic stages. The results with L donovani have shown that infection rates in sand flies can be strain-specific; therefore, to determine vector competence or refractoriness, it is optimal to test at least two strains of Leishmania. CONCLUSIONS, SIGNIFICANCE: Both sand fly species tested are potential vectors of L. donovani and L. major in Mediterranean area. However, further studies will be needed to identify European vectors of L. martiniquensis and to test the ability of other European sand fly species to transmit L. major, L. donovani, L. tropica and L. infantum.
- MeSH
- hmyz - vektory * parazitologie fyziologie MeSH
- kur domácí parazitologie MeSH
- Leishmania * fyziologie klasifikace genetika MeSH
- leishmanióza přenos parazitologie MeSH
- lidé MeSH
- Phlebotomus * parazitologie fyziologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Evropa MeSH
Učební texty
1.vyd. 16 s.
bez vyd. 16 s.
- Klíčová slova
- ošetřovatelská péče,
- MeSH
- diabetes mellitus MeSH
- dítě MeSH
- Check Tag
- dítě MeSH
- MeSH
- dítě MeSH
- fruktosa diagnostické užití MeSH
- vrozené poruchy metabolismu krev MeSH
- Check Tag
- dítě MeSH
16 s. : il. ; 30 cm
- Konspekt
- Lékařské vědy. Lékařství
- NLK Obory
- vnitřní lékařství
- ošetřovatelství
- pediatrie