AIM: Our study aimed to investigate the effect of amlodipine on bone metabolism in orchidectomized rats. METHODS: Eight-week-old rats were divided into three groups. The sham-operated control group (SHAM) and the control group after orchidectomy (ORX) received the standard laboratory diet (SLD). The experimental group after orchidectomy (ORX+AML) received SLD enriched with amlodipine for 12 weeks. Bone marker concentrations in serum of PINP, OPG and IGF-1, and the levels of CTX-I, BAP and BMP-2 in a bone homogenate were measured using enzyme-linked immunosorbent assay. Bone mineral density (BMD) was measured by dual energy X-ray absorptiometry. The femurs were used for biomechanical testing. RESULTS: Bone markers (CTX-I, BAP, BMP-2) in ORX were higher versus SHAM. In ORX+AML there was a decrease in PINP, CTX-I, BAP, BMP-2 and OPG versus ORX. IGF-1 was decreased in ORX versus SHAM. In ORX+AML it was increased versus ORX. In ORX, a decrease was demonstrated versus SHAM in BMD of the whole body, in the lumbar vertebrae and in both femurs. In ORX+AML there was an increase in BMD of the whole body versus ORX. Three-point bending test revealed a decrease in maximal load values in ORX versus SHAM. After amlodipine administration there was an increase in the left femur versus ORX. CONCLUSIONS: Amlodipine is capable of mitigating the negative effects of orchidectomy and could be a good prevention of osteoporosis.
- MeSH
- alkalická fosfatasa metabolismus MeSH
- amlodipin farmakologie terapeutické užití MeSH
- biologické markery krev MeSH
- biomechanika fyziologie MeSH
- blokátory kalciových kanálů farmakologie terapeutické užití MeSH
- insulinu podobný růstový faktor I metabolismus MeSH
- kolagen typu I metabolismus MeSH
- kosti a kostní tkáň účinky léků metabolismus MeSH
- kostní denzita účinky léků MeSH
- kostní morfogenetický protein 2 metabolismus MeSH
- krysa rodu rattus MeSH
- modely nemocí na zvířatech MeSH
- orchiektomie škodlivé účinky MeSH
- osteoporóza farmakoterapie patofyziologie prevence a kontrola MeSH
- osteoprotegerin krev MeSH
- peptidové fragmenty krev MeSH
- potkani Wistar MeSH
- prokolagen krev MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Recent studies have shown that atorvastatin influences bone metabolism. We investigated its bone protective effect in orchidectomised rats after 12 weeks of treatment. Eight-week-old rats were divided into 3 groups: sham-operated group, control group after orchidectomy and experimental group after orchidectomy with atorvastatin administration (12 mg/kg/day). Bone mineral density and bone marker concentrations of aminoterminal propeptide of procollagen type I (PINP), osteoprotegerin (OPG), insulin-like growth factor 1 (IGF-1) in serum, and carboxy-terminal cross-linking telopeptide of type I collagen (CTX-I), bone alkaline phosphatase (BALP), bone morphogenetic protein 2 (BMP-2) in bone homogenate were measured. Total serum calcium and tibial calcium content was determined. Femurs were used for three-point bending test of the shaft and compression testing of the femoral neck. Bone markers (CTX-I, BALP, BMP-2) in control rats were higher vs. sham-operated rats. Atorvastatin reduced CTX-I, BMP-2 and OPG compared to controls. IGF-1 was decreased in control rats vs. sham-operated rats; atorvastatin increased IGF-1 vs. control rats. Atorvastatin exerts a positive effect on bone metabolism by increasing bone mineral density of the whole body, which had decreased under the effects of orchidectomy. Three-point bending test revealed an increase in maximal load values of the left femurs after atorvastatin administration compared to controls. The diameter of the left femur and length of both femurs were increased after atorvastatin administration compared to controls. Our findings suggest that atorvastatin has a beneficial effect on bone metabolism in orchidectomised rats by decreasing bone turnover, with resulting improvement in bone mineral density and bone biomechanical properties.
- MeSH
- biologické markery metabolismus MeSH
- femur účinky léků metabolismus MeSH
- inhibitory kostní resorpce farmakologie terapeutické užití MeSH
- kosti a kostní tkáň metabolismus MeSH
- kostní denzita účinky léků MeSH
- krysa rodu rattus MeSH
- kyseliny heptylové farmakologie terapeutické užití MeSH
- modely nemocí na zvířatech MeSH
- orchiektomie škodlivé účinky MeSH
- osteoporóza farmakoterapie metabolismus MeSH
- pevnost v tlaku účinky léků MeSH
- potkani Wistar MeSH
- pyrroly farmakologie terapeutické užití MeSH
- tibie účinky léků metabolismus MeSH
- vápník krev metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Introduction: We studied influence of mud-bath on bone status in male Wistar rats with subchronic arthritis. Methods: Arthritis was induced by 2 subplantar injections of Freund's adjuvans with heat-killed Streptoccocus pyogenes into paw. Groups: intact (int) on chippings; (con) arthritis on chippings; (san38) arthritis on hot sand; (mu38) arthritis on hot mud; (mu21) arthritis on mild mud. Bone mineral density (BMD, g/cm2) was measured by dual energy X-ray absorptiometry and femurs were tested biomechanically. Bone markers osteocalcin (OC), PINP and CTX were analysed in bone. Results: BMD of right femur decreased vs. left in san38 (p = 0.030) and mu38 (p = 0.047). Fracture load of right/left femur (N) decreased in experimental groups, significantly in san38 (p = 0.05). Fracture threshold of neck decreased in right vs. left in experimental groups, but significantly in san38 (p = 0.05). OC decreased in mu38 vs. con (1.84 ± 0.14/2.62 ± 0.23). PINP decreased in int vs. san38 (p = 0.005) and mu21 (p < 0.001). CTX decreased in int vs. mu38 (p = 0.006) and mu21 (p = 0.005). Conclusion: The hot bath appears indifferent in relation to osteoporosis, while cold mud-bath shows good effect on bone metabolism. The cold mud-baths help to reduce arthritic inflammation and pain and thereby lead to higher mobility with positive consequence on bone.
- MeSH
- artritida experimentální MeSH
- biomechanika MeSH
- experimenty na zvířatech MeSH
- fraktury femuru MeSH
- kosti a kostní tkáň * metabolismus MeSH
- kostní denzita MeSH
- modely nemocí na zvířatech * MeSH
- osteokalcin MeSH
- osteoporóza MeSH
- potkani Wistar MeSH
- statistika jako téma MeSH
- terapie bahnem MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
