- Publikační typ
- abstrakt z konference MeSH
Sepsis is a life threatening condition that arises when the body's response to an infection injures its own tissues and organs. Sepsis can lead to shock, multiple organ failure and death especially if not recognized early and treated promptly. Molecular mechanisms underlying the systemic inflammatory response syndrome associated with sepsis are still not completely defined and most therapies developed to target the acute inflammatory component of the disease are insufficient. In this study we investigated a possibility of combating sepsis in a mouse model by intravenous treatment with recombinant human tissue non-specific alkaline phosphatase (rhTNAP) derived from transgenic rabbit milk. We induced sepsis in mice by intraperitoneal injection of LPS and three hours later treated experimental group of mice by intravenous injection with rhTNAP derived from transgenic rabbits. Such treatment was proved to be physiologically effective in this model, as administration of recombinant rhTNAP successfully combated the decrease in body temperature and resulted in increased survival of mice (80 % vs. 30 % in a control group). In a control experiment, also the administration of bovine intestinal alkaline phosphatase by intravenous injection proved to be effective in increasing survival of mice treated with LPS. Altogether, present work demonstrates the redeeming effect of the recombinant tissue non-specific AP derived from milk of genetically modified rabbits in combating sepsis induced by LPS.
- MeSH
- alkalická fosfatasa terapeutické užití MeSH
- geneticky modifikovaná zvířata MeSH
- králíci MeSH
- lidé MeSH
- lipopolysacharidy toxicita MeSH
- míra přežití trendy MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- pilotní projekty MeSH
- rekombinantní proteiny terapeutické užití MeSH
- sepse chemicky indukované farmakoterapie mortalita MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Klíčová slova
- BCG lymfadenitida, Východočeský kraj,
- MeSH
- BCG vakcína škodlivé účinky MeSH
- lidé MeSH
- longitudinální studie MeSH
- lymfadenitida chemicky indukované terapie MeSH
- lymfatické uzliny patologie MeSH
- novorozenec imunologie MeSH
- tuberkulóza MeSH
- Check Tag
- lidé MeSH
- novorozenec imunologie MeSH
- Geografické názvy
- Česká republika MeSH
