Galektíny patria medzi endogénne lektíny – bielkoviny špecificky rozpoznávajúce cukorné motívy. Galektíny hrajú významné úlohy v procesoch bunkovej proliferácie, diferenciácie, migrácie a tvorby medzibunkovej hmoty. Navyše sú schopné prenášať bunkové signály a podieľať sa na medzibunkových interakciách. Podobne bolo dokázané, že galektíny zohrávajú dôležitú úlohu pri tvorbe mikroprostredia nádoru a/alebo hojacej sa rany. Táto práca poskytuje prehľad experimentálnych a klinických štúdií zaoberajúcich sa biologickými úlohami galektínov v tkanivovej reparácii a jej paralele k raste nádoru.
Galectins are representatives of endogenous lectins – molecules specifically recognizing distinct sugar motifs. They play an important role in the processes of cell proliferation, differentiation, migration and extracellular matrix formation. Furthermore, galectins are able to transfer cellular signals and to participate in intercellular interaction. It has been proven that galectins play an important role in the formation of tumor and/or wound healing microenvironment. This review contains an overview of experimental and clinical studies dealing with biological roles of galectins in tissue repair and in its parallel – the tumor growth.
- MeSH
- exprese genu fyziologie MeSH
- galektin 1 fyziologie metabolismus MeSH
- galektin 3 fyziologie metabolismus MeSH
- galektiny * fyziologie klasifikace metabolismus MeSH
- hojení ran fyziologie MeSH
- lidé MeSH
- modely u zvířat MeSH
- nádory patofyziologie MeSH
- tkáně fyziologie metabolismus MeSH
- výzkum MeSH
- zánět patofyziologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- přehledy MeSH
Previously, we found that treatment of cutaneous wounds with Atropa belladonna L. (AB) revealed shortened process of acute inflammation as well as increased tensile strength and collagen deposition in healing skin wounds (Gál et al. 2009). To better understand AB effect on skin wound healing male Sprague-Dawley rats were submitted to one round full thickness skin wound on the back. In two experimental groups two different concentrations of AB extract were daily applied whereas the control group remained untreated. For histological evaluation samples were removed on day 21 after surgery and stained for wide spectrum cytokeratin, collagen III, fibronectin, galectin-1, and vimentin. In addition, in the in vitro study different concentration of AB extract were used to evaluate differences in HaCaT keratinocytes proliferation and differentiation by detection of Ki67 and keratin-19 expressions. Furthermore, to assess ECM formation of human dermal fibroblasts on the in vitro level fibronectin and galectin-1 were visualized. Our study showed that AB induces fibronectin and galectin-1 rich ECM formation in vitro and in vivo. In addition, the proliferation of keratinocytes was also increased. In conclusion, AB is an effective modulator of skin wound healing. Nevertheless, further research is needed to find optimal therapeutic concentration and exact underlying mechanism of action.
- MeSH
- Atropa belladonna chemie MeSH
- časové faktory MeSH
- extracelulární matrix metabolismus účinky léků MeSH
- fibroblasty metabolismus patologie účinky léků MeSH
- fibronektiny metabolismus MeSH
- galektin 1 metabolismus MeSH
- hojení ran účinky léků MeSH
- keratin-19 metabolismus MeSH
- keratinocyty metabolismus patologie účinky léků MeSH
- kolagen typ III metabolismus MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- kůže metabolismus patologie účinky léků zranění MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- penetrující rány farmakoterapie metabolismus patologie MeSH
- potkani Sprague-Dawley MeSH
- rostlinné extrakty farmakologie chemie izolace a purifikace MeSH
- rozpouštědla chemie MeSH
- vimentin metabolismus MeSH
- voda chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
