Remifentanil is a commonly used opioid in anesthesia with cardioprotective effect in ischemia-reperfused (I/R) heart. We evaluated the influence of remifentanil on myocardial infarct size and expressions of proteins involved in apoptosis in I/R rat heart following various time protocols of remifentanil administration. Artificially ventilated anesthetized Sprague-Dawley rats were subjected to a 30 min of left anterior descending coronary artery occlusion followed by 2 h of reperfusion. Rats were randomly assigned to one of five groups; Sham, I/R only, remifentanil preconditioning, postconditioning and continuous infusion group. Myocardial infarct size, the phosphorylation of ERK1/2, Bcl2, Bax and cytochrome c and the expression of genes influencing Ca2+ homeostasis were assessed. In remifentanil-administered rat hearts, regardless of the timing and duration of administration, infarct size was consistently reduced compared to I/R only rats. Remifentanil improved expression of ERK 1/2 and anti-apoptotic protein Bcl2, and expression of sarcoplasmic reticulum genes which were significantly reduced in the I/R rats only. Remifentanil reduced expression of pro-apoptotic protein, Bax and cytochrome c. These suggested that remifentanil produced cardioprotective effect by preserving the expression of proteins involved in anti-apoptotic pathways, and the expression of sarcoplasmic reticulum genes in I/R rat heart, regardless of the timing of administration.

• MeSH
• adjuvancia anestetická MeSH
• apoptóza účinky léků   MeSH
• časové faktory MeSH
• cytochromy c metabolismus   MeSH
• financování organizované MeSH
• fosforylace MeSH
• hemodynamika účinky léků   MeSH
• homeostáza MeSH
• infarkt myokardu MeSH
• krysa rodu rattus MeSH
• messenger RNA metabolismus   MeSH
• mitogenem aktivovaná proteinkinasa 1 metabolismus   MeSH
• mitogenem aktivovaná proteinkinasa 3 metabolismus   MeSH
• modely nemocí na zvířatech MeSH
• myokard metabolismus   patologie   MeSH
• piperidiny aplikace a dávkování   farmakologie   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• potkani Sprague-Dawley MeSH
• protein X asociovaný s bcl-2 metabolismus   MeSH
• protoonkogenní proteiny c-bcl-2 metabolismus   MeSH
• regulace genové exprese účinky léků   MeSH
• reperfuze myokardu MeSH
• sarkoplazmatické retikulum metabolismus   MeSH
• vápník metabolismus   MeSH
• viabilita buněk účinky léků   MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH