This research paper presents a novel approach to the green synthesis of silver nanoparticles (AgNPs) using viticultural waste, allowing to obtain NP dispersions with distinct properties and morphologies (monodisperse and polydisperse AgNPs, referred to as mAgNPs and pAgNPs) and to compare their biological activities. Our synthesis method utilized the ethanolic extract of Vitis vinifera pruning residues, resulting in the production of mAgNPs and pAgNPs with average sizes of 12 ± 5 nm and 19 ± 14 nm, respectively. Both these AgNPs preparations demonstrated an exceptional stability in terms of size distribution, which was maintained for one year. Antimicrobial testing revealed that both types of AgNPs inhibited either the growth of planktonic cells or the metabolic activity of biofilm sessile cells in Gram-negative bacteria and yeasts. No comparable activity was found towards Gram-positives. Overall, pAgNPs exhibited a higher antimicrobial efficacy compared to their monodisperse counterparts, suggesting that their size and shape may provide a broader spectrum of interactions with target cells. Both AgNP preparations showed no cytotoxicity towards a human keratinocyte cell line. Furthermore, in vivo tests using a silkworm animal model indicated the biocompatibility of the phytosynthesized AgNPs, as they had no adverse effects on insect larvae viability. These findings emphasize the potential of targeted AgNPs synthesized from viticultural waste as environmentally friendly antimicrobial agents with minimal impact on higher organisms.

• MeSH
• Anti-Infective Agents pharmacology   chemistry   MeSH
• Biofilms drug effects   MeSH
• Bombyx MeSH
• Cell Line MeSH
• Gram-Negative Bacteria drug effects   MeSH
• Keratinocytes drug effects   MeSH
• Metal Nanoparticles * chemistry   MeSH
• Yeasts drug effects   MeSH
• Larva drug effects   MeSH
• Humans MeSH
• Microbial Sensitivity Tests * MeSH
• Plant Extracts pharmacology   chemistry   MeSH
• Silver * pharmacology   chemistry   metabolism   MeSH
• Green Chemistry Technology MeSH
• Particle Size MeSH
• Cell Survival drug effects   MeSH
• Vitis * chemistry   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH