The article contains information on the properties of fentanyl derivatives and a method for their simple orienta-tion identification. Thin-layer chromatography (TLC) was deliberately chosen to perform the experiment because it is the most suitable method for rapid analysis of the col-lected sample under the conditions of the field chemical laboratory operated by the Army of the Czech Republic. On the basis of the experimental work performed, it was found that the most suitable system for the identification of the fentanyl derivatives of interest by TLC is a system where the stationary phase consists of "alumina 60 F254 neutral" and the mobile phase is comprised of ethyl ace-tate and hexane in a ratio of 7:3. The findings are fully usable in practice and will be included in the standard operating procedures for the determination of these types of substances in the Field Transport Chemical Laboratory PPCHL-AL2/ch, most likely in the form of acertified methodology.
An integral part of the research and development of new antidotes against nerve agents (herein referred to as NAs) is the verification of their efficacy by means of in vitro tests. The purpose of these tests is to verify the ability of an antidote to protect cholinesterases from inhibition or to reactivate inhibited cholinesterases. For this purpose cholinesterases of different species and with a different degree of purification are traditionally used. Using immobilized cholinesterases proves to be new and advantageous for a number of reasons. The authors of the presented work verified the usability of Detehit detector (Oritest Praha Ltd) containing the pig’s brain acetylcholinesterase for this purpose. In this article we see the results of tests focused on verifying the effect of different reaction conditions on the activity of acetylcholinesterase, especially with regard to reproducibility of these measurements.
- MeSH
- acetylcholinesterasa účinky léků MeSH
- antidota terapeutické užití MeSH
- biosenzitivní techniky metody využití MeSH
- buněčné kultury využití MeSH
- chemické bojové látky škodlivé účinky toxicita MeSH
- mozek enzymologie MeSH
- reaktivátory cholinesterázy terapeutické užití MeSH
- Sus scrofa MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- techniky in vitro MeSH
- Publikační typ
- abstrakt z konference MeSH
