The opportunistic Gram-negative bacterium Burkholderia cenocepacia causes lethal infections in cystic fibrosis patients. Multivalent mannoside derivatives were prepared as potential inhibitors of lectin BC2L-A, one of the virulence factors deployed by B. cenocepacia in the infection process. An (α1→2)-thio-linked mannobioside mimic bearing an azide functionalized aglycon was conjugated to different multivalent scaffolds such as propargylated calix[4]arenes, methyl gallate and pentaerythritol by azide-alkyne 1,3-dipolar cycloaddition. The interaction between the glycoclusters and the mannose binding BC2L-A lectin from B. cenocepacia was examined by isothermal microcalorimetry, surface plasmon resonance, inhibition of yeast agglutination and analytical ultracentrifugation.
- MeSH
- aglutinační testy MeSH
- Burkholderia cenocepacia chemie MeSH
- kalorimetrie metody MeSH
- kvasinky účinky léků MeSH
- lektin vázající mannosu chemie metabolismus farmakologie MeSH
- ligandy MeSH
- mannosidy chemická syntéza chemie metabolismus MeSH
- povrchová plasmonová rezonance MeSH
- techniky syntetické chemie MeSH
- ultracentrifugace metody MeSH
- Publikační typ
- časopisecké články MeSH
