Complex in vitro characterization of a blended material based on Poly(Lactic Acid), Poly(Hydroxybutyrate), and Thermoplastic Starch (PLA/PHB/TPS) was performed in order to evaluate its potential for application in the field of tissue engineering. We focused on the biological behavior of the material as well as its mechanical and morphological properties. We also focused on the potential of the blend to be processed by the 3D printer which would allow the fabrication of the custom-made scaffold. Several blends recipes were prepared and characterized. This material was then studied in the context of scaffold fabrication. Scaffold porosity, wettability, and cell-scaffold interaction were evaluated as well. MTT test and the direct contact cytotoxicity test were applied in order to evaluate the toxic potential of the blended material. Biocompatibility studies were performed on the human chondrocytes. According to our results, we assume that material had no toxic effect on the cell culture and therefore could be considered as biocompatible. Moreover, PLA/PHB/TPS blend is applicable for 3D printing. Printed scaffolds had highly porous morphology and were able to absorb water as well. In addition, cells could adhere and proliferate on the scaffold surface. We conclude that this blend has potential for scaffold engineering.

• MeSH
• 3D tisk MeSH
• hydroxybutyráty farmakologie   terapeutické užití   MeSH
• lidé MeSH
• polyestery farmakologie   terapeutické užití   MeSH
• tkáňové inženýrství metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Shrinkage of ceramic objects produced by Fused Depositon Ceramics 3D printing technology was studied as model procedure for production of biocompatible scaffolds. The formulation of ceramic composite filament tested was based on components such as aluminium and silicium oxides and thermoplastic polymer. The resulting ceramic material after sintering is approaching the chemical composition of the mullite ceramics, which has several interesting material properties. The shrinkage of the produced testing objects was studied as function of the particle content in starting composite and sintering temperature. Observed shrinkage of the ceramic bodies produced was on the level of 17% for 65 weight % and the 23% for 40 weight % of inorganic filler content at temperature 1200 °C, respectively, with well maintained shape. The tested ceramic scaffolds were produced using slice thickness of 0.50 mm and fill gap of 0.58 mm, with regular rectilinear infill pores generated by Slic3r.