Effects of active ingredients from topical and systemic skincare products on structure and organization of epidermis, dermal-epidermal junction (DEJ), and dermis were examined using an in vitro reconstructed skin equivalent (SE). Imedeen Time Perfection (ITP) ingredients (a mixture of BioMarine Complex, grape seed extract, tomato extract, vitamin C) were supplemented systemically into culture medium. Kinetin, an active ingredient from Imedeen Expression Line Control Serum, was applied topically. Both treatments were tested separately or combined. In epidermis, all treatments stimulated keratinocyte proliferation, showing a significant increase of Ki67-positive keratinocytes (P < 0.05). Kinetin showed a twofold increase of Ki67-positive cells, ITP resulted in a fivefold, and ITP+kinetin showed a nine-fold increase. Differentiation of keratinocytes was influenced only by kinetin since filaggrin was found only in kinetin and kinetin+ITP samples. At the DEJ, laminin 5 was slightly increased by all treatments. In dermis, only ITP increased the amount of collagen type I. Both kinetin and ITP stimulated formation of fibrillin-1 and elastin deposition. The effect of kinetin was seen in upper dermis. It stimulated not only the amount of deposited fibrillin-1 and elastin fibers but also their organization perpendicularly to the DEJ. ITP stimulated formation of fibrillin-1 in deeper dermis. In summary, the combination of topical treatment with kinetin and systemic treatment with ITP had complementary beneficial effects in the formation and development of epidermis and dermis.

• MeSH
• antigen Ki-67 metabolismus   MeSH
• aplikace lokální MeSH
• buněčné kultury MeSH
• dospělí MeSH
• elastin metabolismus   MeSH
• epidermis cytologie   MeSH
• glykosaminoglykany aplikace a dávkování   farmakologie   MeSH
• keratinocyty cytologie   metabolismus   MeSH
• kinetin metabolismus   MeSH
• kultivované buňky MeSH
• kůže MeSH
• lékové interakce MeSH
• lidé MeSH
• mikrofilamentové proteiny metabolismus   MeSH
• péče o kůži MeSH
• proteiny intermediálních filament analýza   metabolismus   MeSH
• proteiny aplikace a dávkování   farmakologie   MeSH
• regulátory růstu rostlin farmakologie   MeSH
• škára cytologie   MeSH
• umělá kůže MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• srovnávací studie MeSH