Structural analysis of the orientations of heme vinyl side chains was carried out using the published crystallographic data for different cytochromes P450. Torsional angles (tau, C(alpha)C(beta)-C(a)C(b)) show different distributions for the vinyls in positions 2 and 4. Whereas the orientation of 2-vinyls is rather restricted (tau between -120 degrees and -180 degrees ), the 4-vinyls have a much higher mobility over almost the entire conformational space. On the basis of the empirical correlation recently reported for peroxidases (M.P. Marzocchi, G. Smulevich, Relationship between heme vinyl conformation and the protein matrix in peroxidases, J. Raman Spectrosc. 34 (2003), 725-736), an attempt has been made to compare the observed vinyl orientations with the experimental frequencies of the vinyl stretching vibrational modes. The data for P450 proteins do not exactly match the peroxidase-derived function, although a qualitatively similar relationship is likely to exist. Differences between P450 forms suggest a variability in heme-region flexibility and in communication with the rest of enzyme.
- MeSH
- 5-monooxygenasa kafru chemie MeSH
- bakteriální proteiny chemie MeSH
- financování organizované MeSH
- hem chemie MeSH
- izoenzymy chemie MeSH
- konformace proteinů MeSH
- NADPH-cytochrom c-reduktasa MeSH
- oxygenasy se smíšenou funkcí chemie MeSH
- Ramanova spektroskopie MeSH
- systém (enzymů) cytochromů P-450 chemie MeSH
