The goal of the present study was to investigate the daily rhythms of calcium, phosphate and 1.25 dihydroxyvitamin D3 (1.25-(OH)2-D3) in the blood serum of goats. Blood samples from six Girgentana breed goats were collected via an intravenous cannula inserted into the jugular vein at four-hour intervals over a 48-hour period (starting at 08:00 hours on day 1 and finishing at 04:00 on day 2). The serum concentration of calcium and phosphate was measured by photometric test and of 1.25-(OH) 2-D3 by HPLC. All parameters were expressed as mean ? SEM. The one-way repeated measures analysis of variance (ANOVA) was used to determine significant differences. p values <0.05 were considered statistically significant. ANOVA showed a highly significant effect of time in all the parameters studied (p<0.0001 for calcium and phosphate and p<0.0002 for 1.25-(OH) 2-D3). The application of the periodic model and the statistical analysis of the Cosinor enabled us to define the periodic parameters and their acrophases (expressed in hours) during the 2 days of monitoring: all the studied parameters exhibited diurnal acrophases, which were within 12:44 and 17:28 hours. The results obtained led us to reveal the existence of a daily rhythm for the parameters considered and their temporal physiological values are useful for their implications in the formulation of therapeutic and nutritional protocols in the goat.
- MeSH
- 24,25-dihydroxyvitamin D3 analýza chemie krev MeSH
- cirkadiánní rytmus fyziologie MeSH
- fosforečnany vápenaté analýza chemie krev MeSH
- kozy fyziologie krev MeSH
- sérum fyziologie chemie MeSH
- spektrofotometrie infračervená metody využití MeSH
- vysokoúčinná kapalinová chromatografie metody využití MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
