In screening biocontrol strains with broad-spectrum and high-efficiency herbicidal activities, a strain with strong pathogenicity, HY-021, was isolated from the leaves of Rumex acetosa, which was identified as Botrytis fabiopsis based on morphology and molecular biology. The herbicidal activities of the fermentation filtrate of strain HY-021 against nine weeds, including Chenopodium album L., Elsholtzia densa Benth., Malva verticillata L. var. Crispa, Polygonum lapathifolium L., Amaranthus retroflexus L., Avena fatua L., Thlaspi arvense L., Polygonum aviculare L., and Galium spurium L., were determined in vitro and in vivo. The results showed that the pathogenicity of strain HY-021 to the different weeds in vitro was as follows: E. densa > A. retroflexus > P. aviculare > P. lapathifolium > M. verticillata > T. arvense > G. spurium > A. fatua > C. album. Seven days after inoculation with the HY-021 strain, the incidences in nine weeds were in the range of 32.9-87.23%, and the disease index values of the nine weeds were 41.73-94.57%. The pathogenic effects from high to low were A. retroflexus > E. densa > A. fatua > G. spurium > C. album > M. verticillata > T. arvense > P. aviculare > P. lapathifolium. The crop safety test showed that the biocontrol strain HY-021 was safe to V. faba, P. sativum, H. vulgare, and T. aestivum, but had a slight effect on B. napus. Scanning electron microscopy showed that the mycelium of strain HY-021 invaded the tissue through the stomata of C. album leaves, parasitized and reproduced in the tissue, and gradually destroyed the tissue. The results of this study provide a basis for the development and utilization of new and efficient microbial source herbicides.
- MeSH
- Pest Control, Biological * methods MeSH
- Biological Control Agents * MeSH
- Botrytis * isolation & purification physiology genetics pathogenicity MeSH
- Herbicides metabolism pharmacology MeSH
- Weed Control * methods MeSH
- Plant Leaves microbiology MeSH
- Plant Diseases * microbiology prevention & control MeSH
- Plant Weeds * microbiology MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- China MeSH
- MeSH
- Allergens MeSH
- Alternaria immunology isolation & purification pathogenicity MeSH
- Aspergillus immunology isolation & purification pathogenicity MeSH
- Botrytis immunology isolation & purification pathogenicity MeSH
- Cladosporium immunology isolation & purification pathogenicity MeSH
- Financing, Organized MeSH
- Fusarium immunology isolation & purification pathogenicity MeSH
- Helminthosporium immunology isolation & purification pathogenicity MeSH
- Fungi classification MeSH
- Humans MeSH
- Air Microbiology MeSH
- Environmental Monitoring MeSH
- Penicillium immunology isolation & purification pathogenicity MeSH
- Spores, Fungal classification MeSH
- Environment MeSH
- Air Pollution adverse effects MeSH
- Check Tag
- Humans MeSH
- Geographicals
- Czech Republic MeSH
