OBJECTIVES: Currently, many questions regarding the effect of anaesthetics to fish remain unresolved. Fish species may differ widely in their response to an anaesthetic, the screening of dosages is often necessary. The aim of this study was to compare the effect of tricaine methane sulphonate (MS 222), clove oil, 2-phenoxyethanol and Propiscin on haematological profiles, oxidative stress biomarkers and antioxidant enzymes in barbel (Barbus barbus). DESIGN: The haematological profiles, oxidative stress biomarkers and antioxidant enzymes of barbel were evaluated immediately after a 10 min anaesthesia (MS 222--100 mg.L(-1), clove oil--33 mg.L(-1), 2-phenoxyethanol--0.4 mg.L(-1), Propiscin--1.0 mg.L(-1)), and 24 h after anaesthesia. RESULTS: The 10 min exposure in the recommended concentrations of tested anaesthetics have no significant effect on haematological profiles, levels of thiobarbituric acid reactive substances, and activity of glutathione reductase of barbel. The activity of superoxide dismutase (SOD) was significantly decreased (p<0.01) in the muscle in all experimental groups. The activity of SOD showed a significant decrease (p<0.01) in the liver 24 h after all anaesthetics; however in the gill the activity of SOD was significantly increased (p<0.01) in Propiscin (10 min). The activity of catalase (CAT) was significantly increased (p<0.05) in the muscle 24 h after all anaesthetics. CONCLUSIONS: The observed effects on barbel antioxidant systems may be a defence against oxidative damage. The results of this study suggest that the antioxidant systems of barbel are altered by Propiscin anaesthesia, but are slightly affected by MS 222, clove oil, and 2-phenoxyethanol anaesthesia.

• MeSH
• aminobenzoáty farmakologie   MeSH
• anestetika farmakologie   MeSH
• Cyprinidae MeSH
• ethylenglykoly farmakologie   MeSH
• etomidát farmakologie   MeSH
• glutathionreduktasa účinky léků   metabolismus   MeSH
• hřebíčkový olej farmakologie   MeSH
• játra účinky léků   enzymologie   MeSH
• kosterní svaly účinky léků   enzymologie   MeSH
• látky reagující s kyselinou thiobarbiturovou metabolismus   MeSH
• oxidační stres účinky léků   MeSH
• superoxiddismutasa účinky léků   metabolismus   MeSH
• žábry účinky léků   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

• Klíčová slova
• Gynipral, Nizoral, Anavenol,
• MeSH
• agonisté beta-2-adrenergních receptorů farmakologie   škodlivé účinky   terapeutické užití   MeSH
• anthrachinony farmakologie   škodlivé účinky   terapeutické užití   MeSH
• aplikace orální MeSH
• ethylenglykoly farmakologie   terapeutické užití   MeSH
• ketokonazol farmakologie   škodlivé účinky   terapeutické užití   MeSH
• kontrola léčiv a omamných látek MeSH
• léčivé přípravky * normy   MeSH
• lidé MeSH
• nežádoucí účinky léčiv MeSH
• stažení léku z trhu z bezpečnostních důvodů * MeSH
• těhotenství účinky léků   MeSH
• Check Tag
• lidé MeSH
• těhotenství účinky léků   MeSH
• ženské pohlaví MeSH

• MeSH
• ethylenglykoly * farmakologie   škodlivé účinky   terapeutické užití   MeSH
• lidé MeSH
• námelové alkaloidy škodlivé účinky   MeSH
• nežádoucí účinky léčiv MeSH
• stažení léku z trhu z bezpečnostních důvodů * MeSH
• Check Tag
• lidé MeSH

OBJECTIVES: The objectives of the study were to compare the effects of Propiscin, 2-phenoxyethanol, clove oil and tricaine methane sulphonate (MS 222), anaesthetics frequently used in aquaculture. DESIGN: The haematological and biochemical blood profiles of pikeperch (Sander lucioperca L.) anesthetized with Propiscin (1.5 ml L-1), 2-phenoxyethanol (0.3 ml L-1), clove oil (33 mg L-1), MS 222 (150 mg L-1) and non-anesthetized control group were tested. Each tested group was divided into two subgroups, the first subgroup was sampled in anaesthesia 10 min after application of the anaesthetic and the second one live on 24h. RESULTS: The erythrocyte count and haematocrit was significantly decreased in 2-phenoxyethanol (24 h) compared with control group (CG). The mean corpuscular haemoglobin concentration was significantly increased in 2-phenoxyethanol (10 min), Propiscin (10 min and 24 h) compared to CG. The 2-phenoxyethanol (10 min and 24 h), MS 222 (24 h), clove oil (24 h), and Propiscin (10 min and 24 h) showed significantly lower leukocyte count compared with CG. The level of glucose was significantly (p<0.05) elevated with MS 222 (10 min) and clove oil (10 min) compared with CG. The 2-phenoxyethanol (10 min and 24 h), MS 222 (24 h), clove oil (24 h), and Propiscin (24 h) showed significantly lower (p<0.01) ammonia levels compared with CG. The triacylglycerols was significantly decreased (p<0.01) with Propiscin (10 min and 24 h), MS 222 (24 h), clove oil (24 h) and with 2-phenoxyethanol (24 h) compared with CG. After 24 hours MS 222 (24 h) and Propiscin (24 h) anaesthesia, fish showed significantly lower (p<0.01) concentration of inorganic phosphate compared with CG. CONCLUSIONS: On the basis of this experiment, it appears that clove oil was associated with the lowest effects in pikeperch and therefore would be recommended as an alternative to MS 222, while Propiscin and 2-phenoxyethanol are not suitable for manipulation with pikeperch in aquaculture.

• MeSH
• aminobenzoáty toxicita   MeSH
• anestetika farmakologie   toxicita   MeSH
• Esocidae krev   MeSH
• ethylenglykoly farmakologie   toxicita   MeSH
• etomidát farmakologie   toxicita   MeSH
• hematokrit MeSH
• hřebíčkový olej farmakologie   toxicita   MeSH
• krevní proteiny metabolismus   MeSH
• okounovití krev   MeSH
• počet erytrocytů MeSH
• vodní hospodářství metody   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH